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Use of atomic force microscopy in UVB-induced chromosome damage provides important bioinformation for cell damage assessment

  • Bowei Wang
    ,
  • Jianjun Dong
    ,
  • Fan Yang
    ,
  • Tuoyu Ju
    ,
  • Jiani Li
    ,
  • Junxi Wang
  • Changchun University of Science and Technology
    ,
  • University of Warwick
Research Output: Contribution to journal Article Peer-review

Open access

Sustainable Development Goals

  • SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well

Abstract

The chromosomal structure derived from UVB-stimulated HaCaT cells was detected by atomic force microscopy (AFM) to evaluate the effect of UVB irradiation. The results showed that the higher the UVB irradiation dose, the more the cells that had chromosome aberration. At the same time, different representative types of chromosome structural aberrations were investigated. We also revealed damage to both DNA and cells under the corresponding irradiation doses. It was found that the degree of DNA damage was directly proportional to the irradiation dose. The mechanical properties of cells were also changed after UVB irradiation, suggesting that cells experienced a series of chain reactions from inside to outside after irradiation. The highresolution imaging of chromosome structures by AFM after UVB irradiation enables us to relate the damage between chromosomes, DNA, and cells caused by UVB irradiation and provides specific information on genetic effects.

Publication Information

Output type

Research Output: Contribution to journal Article Peer-review

Original language

English

Pages from-to (Number of pages)

Pages 13212-13221 (10 pages)

Journal (Volume, Issue Number)

Langmuir (Volume 39, Issue 37)

Publication milestones

  • Accepted/In press - 29/08/2023
  • Published - 08/09/2023

Publication status

Published - 08/09/2023

ISSN

0743-7463

External Publication IDs

  • handle.net: 10547/626012
  • Scopus: 85171806753

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