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TCR-alpha CDR3 loop audition regulates positive selection

  • Anna Furmanski
    ,
  • Cristina Ferreira
    ,
  • Maggie M. Millrain
    ,
  • Istvan Bartok
    ,
  • Philippe Guillaume
    ,
  • Rosemary Lees
Research Output: Contribution to journal Article Peer-review

Abstract

How positive selection molds the T cell repertoire has been difficult to examine. In this study, we use TCR-β-transgenic mice in which MHC shapes TCR-α use. Differential AV segment use is directly related to the constraints placed on the composition of the CDR3 loops. Where these constraints are low, efficient selection of αβ pairs follows. This mode of selection preferentially uses favored AV-AJ rearrangements and promotes diversity. Increased constraint on the α CDR3 loops leads to inefficient selection associated with uncommon recombination events and limited diversity. Further, the two modes of selection favor alternate sets of AJ segments. We discuss the relevance of these findings to the imprint of self-MHC restriction and peripheral T cell activation.

Publication Information

Output type

Research Output: Contribution to journal Article Peer-review

Original language

English

Pages from-to (Number of pages)

Pages 2477-2485

Journal (Volume, Issue Number)

Journal of Immunology (Volume 177, Issue 4)

Publication milestones

  • Accepted/In press - 18/05/2006
  • Published - 03/08/2006

Publication status

Published - 03/08/2006

ISSN

0022-1767

External Publication IDs

  • handle.net: 10547/623398
  • Scopus: 33746878837

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