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Study of NSCLC cell migration promoted by NSCLC-derived extracellular vesicle using atomic force microscopy

  • Shuwei Wang
    ,
  • Jiajia Wang
    ,
  • Tuoyu Ju
    ,
  • Fan Yang
    ,
  • Kaige Qu
    ,
  • Wei Liu
  • The First Hospital of Jilin University
    ,
  • Changchun University of Science and Technology
Research Output: Contribution to journal Article Peer-review

Open access

Sustainable Development Goals

  • SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well

Abstract

Extracellular vesicles (EVs) secreted by cancer cells play a key role in the cancer microenvironment and progression. Previous studies have mainly focused on molecular functions, cellular components and biological processes using chemical and biological methods. However, whether the mechanical properties of cancer cells change due to EVs remains poorly understood. This work studies the effects of mechanical changes in non-small cell lung cancer (NSCLC) cells after treatment with EVs on migration by atomic force microscopy (AFM). Different concentrations of EVs were added into the experimental groups based on co-culture experiments, while the control group was cultured without EVs for 48 h. Cellular migration was evaluated by wound healing experiments. The cellular morphology, cell stiffness and surface adhesion were investigated by AFM. Cytoskeleton changes were detected by fluorescence staining assay. By comparison to the control group, the cell migration was enhanced. After treatment with EVs, the cell length and height show an upward trend, and the adhesion force and Young's modulus show a downward trend, and filopodia were also detected in the cells. Overall, the EVs promoted the migration of NSCLC cells by regulating cells' physical properties and skeletal rearrangement.

Publication Information

Output type

Research Output: Contribution to journal Article Peer-review

Original language

English

Pages from-to (Number of pages)

Pages 1455-1462 (8 pages)

Journal (Volume, Issue Number)

Analytical Methods (Volume 13, Issue 12)

Publication milestones

  • Accepted/In press - 15/02/2021
  • Published - 16/02/2021

Publication status

Published - 16/02/2021

ISSN

1759-9660

External Publication IDs

  • handle.net: 10547/624914
  • Scopus: 85103647705

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