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Purification and identification of novel xanthine oxidase inhibitory peptides derived from round scad (Decapterus maruadsi) protein hydrolysates

  • Xiao Hu
    ,
  • Ya Zhou
    ,
  • Shaobo Zhou
    ,
  • Shengjun Chen
    ,
  • Yanyan Wu
    ,
  • Laihao Li
  • Chinese Academy of Fishery Sciences
    ,
  • Jiangsu Ocean University
    ,
  • Shanghai Ocean University
    ,
  • Collaborative Innovation Center of Provincial and Ministerial Co-Construction for Marine Food Deep Processing
Research Output: Contribution to journal Article Peer-review

Open access

Abstract

The objective of the present study was to investigate the xanthine oxidase (XO) inhibitory effects of peptides purified and identified from round scad (Decapterus maruadsi) hydrolysates (RSHs). In this study, RSHs were obtained by using three proteases (neutrase, protamex and alcalase). Among them, the RSHs of 6-h hydrolysis by neutrase displayed the strongest XO inhibitory activity and had an abundance of small peptides (<500 Da). Four novel peptides were purified by immobilized metal affinity chromatography and identified by nano-high-performance liquid chromatography mass/mass spectrometry. Their amino acid sequences were KGFP (447.53 Da), FPSV (448.51 Da), FPFP (506.59 Da) and WPDGR (629.66 Da), respectively. Then the peptides were synthesized to evaluate their XO inhibitory activity. The results indicated that the peptides of both FPSV (5 mM) and FPFP (5 mM) exhibited higher XO inhibitory activity (22.61 +- 1.81% and 20.09 +- 2.41% respectively). Fluorescence spectra assay demonstrated that the fluorescence quenching mechanism of XO by these inhibitors (FPSV and FPFP) was a static quenching procedure. The study of inhibition kinetics suggested that the inhibition of both FPSV and FPFP was reversible, and the type of their inhibition was a mixed one. Molecular docking revealed the importance of π-π stacking between Phe residue (contained in peptides) and Phe914 (contained in the XO) in the XO inhibitory activity of the peptides.

Publication Information

Output type

Research Output: Contribution to journal Article Peer-review

Original language

English

Article number

538

Pages from-to (Number of pages)

Pages 538

Journal (Volume, Issue Number)

Marine Drugs (Volume 19, Issue 10)

Publication milestones

  • Accepted/In press - 21/09/2021
  • Published - 24/09/2021

Publication status

Published - 24/09/2021

External Publication IDs

  • handle.net: 10547/625106
  • Scopus: 85116938814
  • PubMed: 34677437

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