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Proteomic analysis of age-related changes in ovine cerebrospinal fluid

  • Carl P.C. Chen
    ,
  • Jane E. Preston
    ,
  • Shaobo Zhou
    ,
  • Heidi R. Fuller
    ,
  • David G.A. Morgan
    ,
  • Ruoli Chen
  • King's College London
    ,
  • Chang Gung University
    ,
  • Keele University
    ,
  • The Robert Jones and Agnes Hunt Orthopaedic and District Hospital NHS Trust
Research Output: Contribution to journal Article Peer-review

Open access

Abstract

Cerebrospinal fluid (CSF) circulates through the brain and has a unique composition reflecting the biological processes of the brain. Identifying ageing CSF biomarkers can aid in understanding the ageing process and interpreting CSF protein changes in neurodegenerative diseases. In this study, ovine CSF proteins from young (1-2 year old), middle aged (3-6 year old) and old (7-10 year old) sheep were systemically studied. CSF proteins were labelled with iTRAQ tagging reagents and fractionated by 2-dimensional high performance, liquid chromatography. Tryptic peptides were identified using MS/MS fragmentation ions for sequencing and quantified from iTRAQ reporter ion intensities at m/z 114, 115, 116 and 117. Two hundred thirty one peptides were detected, from which 143 proteins were identified. There were 52 proteins with >25% increase in concentrations in the old sheep compared to the young. 33 of them increased >25% but <50%, 13 increased >50% but <1 fold, 6 increased >1 fold [i.e. haptoglobin (Hp), haemoglobin, neuroendocrine protein 7B2, IgM, fibrous sheath interacting protein 1, vimentin]. There were 18 proteins with >25% decrease in concentrations in the old sheep compared to the young. 17 of them decreased >25% but <50%, and histone deacetylase 7 (HDAC7) was gradually decreased for over 80%. Glutathione S-transferase was decreased in middle aged CSF compared to both young and old CSF. The differential expressions of 3 proteins (Hp, neuroendocrine protein 7B2, IgM) were confirmed by immunoassays. These data expand our current knowledge regarding ovine CSF proteins, supply the necessary information to understand the ageing process in the brain and provide a basis for diagnosis of neurodegenerative diseases.

Publication Information

Output type

Research Output: Contribution to journal Article Peer-review

Original language

English

Pages from-to (Number of pages)

Pages 181-188

Journal (Volume, Issue Number)

Experimental Gerontology (Volume 108)

Publication milestones

  • Published - 25/04/2018

Publication status

Published - 25/04/2018

ISSN

0531-5565

External Publication IDs

  • handle.net: 10547/622669
  • Scopus: 85046108196

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