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Osmotic upshift transiently inhibits uptake via ABC transporters in gram-negative bacteria

  • Arthur Hosie
    ,
  • M. A. Fox
    ,
  • James P. White
    ,
  • Emma M. Lodwig
    ,
  • Philip S. Poole
  • University of Reading
Research Output: Contribution to journal Article Peer-review

Abstract

ATP-binding cassette transporters from several rhizobia and Salmonella enterica serovar Typhimurium, but not secondarily coupled systems, were inhibited by high concentrations (100 to 500 mM) of various osmolytes, an effect reversed by the removal of the osmolyte. ABC systems were also inactivated in isolated pea bacteroids, probably due to the obligatory use of high-osmolarity isolation media. Measurement of nutrient cycling in isolated pea bacteroids is impeded by this effect.

Publication Information

Output type

Research Output: Contribution to journal Article Peer-review

Original language

English

Pages from-to (Number of pages)

Pages 5304-5307

Journal (Volume, Issue Number)

Journal of Bacteriology (Volume 188, Issue 14)

Publication milestones

  • Published - 01/01/2006

Publication status

Published - 01/01/2006

ISSN

0021-9193

External Publication IDs

  • handle.net: 10547/293968
  • Scopus: 33745912239

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