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Optimized degradation and inhibition of α-glucosidase activity by gracilaria lemaneiformis polysaccharide and its production in vitro

  • Xiaoshan Long
    ,
  • Xiao Hu
    ,
  • Shaobo Zhou
    ,
  • Huan Xiang
    ,
  • Shengjun Chen
    ,
  • Laihao Li
  • Chinese Academy of Fishery Sciences
    ,
  • Jiangsu Ocean University
    ,
  • Guangdong Ocean University
    ,
  • Collaborative Innovation Center of Provincial and Ministerial Co-Construction for Marine Food Deep Processing
Research Output: Contribution to journal Article Peer-review

Open access

Abstract

Gracilaria lemaneiformis polysaccharide (GLP) exhibits good physiological activities, and it is more beneficial as it is degraded. After its degradation by hydrogen peroxide combined with vitamin C (H2 O2-Vc) and optimized by Box–Behnken Design (BBD), a new product of GLP-HV will be generated. While using GLP as control, two products of GLP-H (H2 O2-treated) and GLP-V (Vc-treated) were also produced. These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed. The results showed that the optimal conditions for H2 O2-Vc degradation were as follows: H2 O2-Vc concentration was 18.7 mM, reaction time was 0.5 h, and reaction temperature was 56◦ C. The total sugar content of GLP and its degradation products (GLP-HV, GLP-H and GLP-V) were more than 97%, and their monosaccharides are mainly glucose and galactose. The SEM analysis demonstrated that H2 O2-Vc made the structure loose and broken. Moreover, GLP, GLP-HV, GLP-H, and GLP-V had significantly inhibition effect on α-glucosidase, and their IC50 value were 3.957, 0.265, 1.651, and 1.923 mg/mL, respectively. GLP-HV had the best inhibition effect on α-glucosidase in a dose-dependent manner, which was the mixed type of competitive and non-competitive. It had a certain quenching effect on fluorescence of α-glucosidase, which may be dynamic quenching.

Publication Information

Output type

Research Output: Contribution to journal Article Peer-review

Original language

English

Article number

13

Pages from-to (Number of pages)

Pages 13

Journal (Volume, Issue Number)

Marine Drugs (Volume 20, Issue 1)

Publication milestones

  • Accepted/In press - 20/12/2021
  • Published - 22/12/2021

Publication status

Published - 22/12/2021

External Publication IDs

  • handle.net: 10547/625290
  • Scopus: 85121660902

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