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Non-destructive genome skimming for aquatic copepods

  • Vinod Vakati
    ,
  • Steven Dodsworth
  • Neijiang Normal University
    ,
  • Hanyang University
Research Output: Contribution to journal Article Peer-review

Open access

Sustainable Development Goals

  • SDG 14 - Life Below Water
    SDG 14 Life Below Water
  • SDG 15 - Life on Land
    SDG 15 Life on Land

Abstract

Copepods are important ecologically and represent a large amount of aquatic biomass in both freshwater and marine systems. Despite this, the taxonomy of copepods and other meiofauna is not well understood, hampered by tiny sizes, cryptic taxa, intraspecific polymorphisms and total specimen destruction where DNA methods are employed. In this article we highlight these issues and propose a more up-to-date approach for dealing with them. Namely, we recommend non-destructive DNA extraction methods, coupled with high-throughput sequencing (HTS). Whilst DNA yields may be low, they should still be sufficient for HTS library preparation and DNA sequencing. At the same time morphological specimens can be preserved and the crucial link between morphology and DNA sequence is maintained. This is critical for an integrative taxonomy and a fuller understanding of biodiversity patterns as well as evolutionary processes in meiofauna.

Publication Information

Output type

Research Output: Contribution to journal Article Peer-review

Original language

English

Pages from-to (Number of pages)

Pages 515-520

Journal (Volume, Issue Number)

Conservation Genetics Resources (Volume 12)

Publication milestones

  • Accepted/In press - 05/01/2020
  • Published - 21/01/2020

Publication status

Published - 21/01/2020

ISSN

1877-7252

External Publication IDs

  • handle.net: 10547/623947
  • Scopus: 85078170210

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