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Migration of BEAS-2B cells enhanced by H1299 cell derived-exosomes

  • Shuwei Wang
    ,
  • Tuoyu Ju
    ,
  • Jiajia Wang
    ,
  • Fan Yang
    ,
  • Kaige Qu
    ,
  • Wei Liu
  • Jilin University
    ,
  • Changchun University of Science and Technology
Research Output: Contribution to journal Article Peer-review

Open access

Sustainable Development Goals

  • SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well

Abstract

Previous studies reported that exosomes (Exos) secreted by tumor cells could affect the tumor cells themselves and normal cells. However, the effects of exosomes derived from tumor cells on normal cells’ migration and mechanical characteristics are rarely reported. This work explores the effects of H1299 cell-derived exosomes (H1299-Exos) on the migration of BEAS-2B cells, and analyzes possible mechanical mechanisms. In the experiments, exosomes were isolated from the culture supernatants of H1299 cells by ultracentrifugation. The H1299-Exos were confirmed by scanning electron microscope (SEM) and western blotting (WB). The BEAS-2B cell migration was assessed using scratch assays. Cytoskeletal structure changes were detected by immunofluorescence. Surface morphology and mechanical properties were measured by atomic force microscopy (AFM). After incubation with H1299-Exos for 48 h, BEAS-2B cells enhanced migration ability, with increased filopodia and cytoskeletal rearrangements. The changes in the morphology and mechanical properties of the cells caused by H1299-Exos were detected using AFM, including the increase in cell length and the decrease in cell height, Young's modulus and adhesion. In short, H1299-Exos promoted the BEAS-2B cell migrations. It indicates that the morphological and mechanical properties can be used as a means to assess normal cell alterations induced by tumor cell derived-exosomes. This provides a method for studying the effects of exosomes secreted by tumor cells on normal cells and the changes in their physical properties.

Publication Information

Output type

Research Output: Contribution to journal Article Peer-review

Original language

English

Article number

103001

Pages from-to (Number of pages)

Pages 103001

Journal (Volume, Issue Number)

Micron (Volume 143)

Publication milestones

  • Accepted/In press - 16/12/2020
  • Published - 12/01/2021

Publication status

Published - 12/01/2021

ISSN

0968-4328

External Publication IDs

  • handle.net: 10547/624882
  • Scopus: 85099862777

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