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Investigation into antiepileptic effect of ganoderic acid A and its mechanism in seizure rats induced by pentylenetetrazole

  • Wei Pang
    ,
  • Shuqing Lu
    ,
  • Rong Zheng
    ,
  • Xin Li
    ,
  • Shunbo Yang
    ,
  • Yuxia Feng
  • Jiamusi University
    ,
  • Qilu Hospital of Shandong University
Research Output: Contribution to journal Article Peer-review

Open access

Abstract

Ganoderic acid A (GAA) exhibited neuron protection in in vitro epilepsy study, but no study has been done in vivo. Rats were administered (i.p.) pentylenetetrazole daily for 28 days to induce seizure. Rats with grade II or above of epileptic score were divided into three groups and given placebo, sodium valproate, or GAA treatment, respectively, for 7 days. The electrical signals of brain were monitored with electroencephalography (EGG); epileptic behavior was assessed using the Racine scale; morphological changes and apoptosis rate of cortical neurons were assessed with H&E staining and TUNEL staining, respectively. Protein expression of calcium-sensing receptor, p-ERK, p-JNK, and p-p38 in hippocampal tissue and Bcl-2, cleaved caspase-3, and Bax in cortical tissues was observed by Western blot and immunohistochemistry assay, respectively. After GAA treatment, apparent seizure-like EEG with significant arrhythmic disorder and spike waves was reduced or disappeared, and wave amplitude of EEG was reduced significantly. GAA showed similar effect with sodium valproate treatments on epilepsy. There were an apparent improvement of the epileptic behavior and a significant increase in the epileptic latency and shortening of the epileptic duration in the treatment group compared to control. GAA treatment ameliorated the nuclear pyknosis of neurons which appeared seriously in the epilepsy group. GAA treatment significantly reduced the cortical neuron apoptosis of epilepsy and the expression of calcium-sensing receptor, p-P38, p-JNK, cleaved caspase-3, and Bax but increased the expression of both p-ERK and Bcl-2. In conclusion, GAA treatment showed strong antiepileptic effect by decreasing apoptosis in cortical neuron and the expression of calcium-sensing receptor and stimulating the MAPK pathway.

Publication Information

Output type

Research Output: Contribution to journal Article Peer-review

Original language

English

Article number

5940372

Pages from-to (Number of pages)

Pages 5940372

Journal (Volume, Issue Number)

BioMed Research International (Volume 2022)

Publication milestones

  • Accepted/In press - 14/06/2022
  • Published - 01/09/2022

Publication status

Published - 01/09/2022

ISSN

2314-6133

External Publication IDs

  • handle.net: 10547/625528
  • PubMed: 36093409
  • Scopus: 85137740982

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