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Investigating effects of silicon nanowire and nanohole arrays on fibroblasts via AFAM

  • Yan Liu
    ,
  • Li Li
    ,
  • Yang Yang
    ,
  • Liguo Tian
    ,
  • Xiaomin Wu
    ,
  • Zhankun Weng
  • Changchun University of Science and Technology
    ,
  • Changchun Medical College
    ,
  • Jilin University
Research Output: Contribution to journal Article Peer-review

Abstract

Understanding the cell–substrate interactions has great significance in tissue regeneration therapies. However, the cell–substrate interactions are not well understood because the interface of cell–substrate is typically buried beneath the cells. This research investigated the subsurfaces of fibroblasts cultured on hybrid nanoarrays using atomic force acoustic microscopy (AFAM). We fabricated hybrid silicon nanowires (SiNWs) and silicon nanoholes (SiNHs) on Si substrates to serve as biomimetic nanoarrays by employing laser interference lithography and the metal-assisted chemical etching (MacEtch) method. After the L929 cells were cultured on the nanoarrays, scanning electron microscopy (SEM) and AFAM were employed to investigate the surface and subsurface of L929 cells. It was suggested that fibroblasts could sense the morphology of the hybrid nanoarrays and membrane damage of fibroblasts on the hybrid nanoarrays were related to the nanostructures. This study can help guide the design of biointerfaces and provide a useful tool for the study of cell subsurfaces in diverse biological fields.

Publication Information

Output type

Research Output: Contribution to journal Article Peer-review

Original language

English

Pages from-to (Number of pages)

Pages 3717-3724

Journal (Volume, Issue Number)

Applied Nanoscience (Switzerland) (Volume 10)

Publication milestones

  • Published - 30/07/2020

Publication status

Published - 30/07/2020

ISSN

2190-5509

External Publication IDs

  • handle.net: 10547/624391
  • Scopus: 85088809203

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