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Influence of magnetic field on morphological structures and physiological characteristics of bEnd.3 cells cultured on polypyrrole substrates

  • Xue Yang
    ,
  • Ke Ma
    ,
  • Libo Yang
    ,
  • Yujuan Chen
    ,
  • Yingmin Qu
    ,
  • Ying Wang
  • Changchun University of Science and Technology
Research Output: Contribution to journal Article Peer-review

Open access

Abstract

This paper employs a spin-coated method to construct conductive polypyrrole (PPy) substrates which present superior properties for controlling the morphological structures and functions of bEnd.3 cells. The PPy substrates with a homogeneous particle size, uniform distribution and proper roughness show enhanced hydrophilic characteristics and improve cell adhesion to the substrates. The changes in the mechanical properties of cells and the responses to the designed substrates and magnetic field are also explored. Due to the synergistic effect between the magnetic field and the conductive PPy substrate, the cells cultured in such an environment exhibit applanate shapes with more branches and enhanced cell viability. In addition, the cells preferentially extend along the magnetic field direction. The mechanical characteristics of cells change significantly under varying magnetic intensity stimulations (5–16 mT). The satisfying effect on cells' morphology and outgrowth is acquired at the magnetic intensities of 9–10 mT and duration of 20 min, compared with other stimulated groups, while retaining cell viability. Moreover, the cells express higher adhesion up to 5.2 nN. The results suggest that the application of the PPy substrates and magnetic field is a promising candidate for the protection of neurovascular units and treatment of neurological diseases.

Publication Information

Output type

Research Output: Contribution to journal Article Peer-review

Original language

English

Pages from-to (Number of pages)

Pages 40887-40894

Journal (Volume, Issue Number)

RSC Advances (Volume 9, Issue 70)

Publication milestones

  • Accepted/In press - 27/11/2019
  • Published - 11/12/2019

Publication status

Published - 11/12/2019

External Publication IDs

  • handle.net: 10547/624094
  • Scopus: 85076706501

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