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Improved DNA straightening and attachment via optimal Mg2+ ionic bonding under electric field for AFM imaging in liquid phase

  • Ziya Liu
    ,
  • Hongmei Xu
    ,
  • Ying Wang
    ,
  • Fan Yang
    ,
  • Yaoting Yin
    ,
  • Sheng Zhang
  • Changchun University of Science and Technology
Research Output: Contribution to journal Article Peer-review

Abstract

In this research, a novel method is proposed to improve DNA straightening under an applied electric field to facilitate imaging in a liquid phase by modifying the substrate with varying Mg2+ ion concentrations. A two-dimensional network of DNA structures was successfully stretched on Mg2+-modified mica substrates under a DC electric field (1 V, 1 A) and imaged in gaseous and aqueous phases by atomic force microscopy. The results revealed that an optimum concentration of Mg2+ ion (4.17 μmol/ml) allowed DNA straightening under an electric field, thus facilitating its imaging in the liquid phase. Furthermore, DNA adhesion under different concentrations of Mg2+ was measured and a maximum adhesion force of 76.19 pN was achieved. This vital work has great potential in gene knockout and targeted gene editing.

Publication Information

Output type

Research Output: Contribution to journal Article Peer-review

Original language

English

Pages from-to (Number of pages)

Pages 102678

Journal (Volume, Issue Number)

Micron (Volume 124)

Publication milestones

  • Accepted/In press - 28/04/2019
  • Published - 25/05/2019

Publication status

Published - 25/05/2019

ISSN

0968-4328

External Publication IDs

  • handle.net: 10547/623755
  • Scopus: 85066864398

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