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Imaging the substructures of individual IgE antibodies with atomic force microscopy

  • Jing Hu
    ,
  • Mingyan Gao
    ,
  • Ying Wang
    ,
  • Mengnan Liu
    ,
  • Jianfei Wang
    ,
  • Jiani Li
  • Changchun University of Science and Technology
Research Output: Contribution to journal Article Peer-review

Abstract

The interaction between antibodies and substrates directly affects its conformation and thus its immune function. Therefore, it is desirable to study the structure of antibodies at the single molecule level. Herein, the substructures of Immunoglobulin E (IgE) on solid surfaces were investigated. For this purpose, the tapping-mode atomic force microscopy (AFM) was applied to observe the individual IgE substructures adsorbed onto Mg2+ and Na+ modified mica substrates in air. As expected, the AFM images revealed that the IgE antibodies exhibited different conformations on the surface of mica substrate, consisting of the four basic orientations: three domain, two equivalent domain, two unequal domain and single domain morphologies. Moreover, the differences of the different orientations in single IgE antibodies were also identified clearly.

Publication Information

Output type

Research Output: Contribution to journal Article Peer-review

Original language

English

Pages from-to (Number of pages)

Pages 14896-14901

Journal (Volume, Issue Number)

Langmuir (Volume 35, Issue 46)

Publication milestones

  • Accepted/In press - 23/10/2019
  • Published - 29/10/2019

Publication status

Published - 29/10/2019

ISSN

0743-7463

External Publication IDs

  • handle.net: 10547/623801
  • Scopus: 85074879834

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