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Identification of the antigens recognised by colorectal cancer patients using sera from patients who exhibit a Crohn's-like lymphoid reaction

  • Viktoriya Boncheva
    ,
  • Michael Linnebacher
    ,
  • Said Kdimati
    ,
  • Hannah Draper
    ,
  • Laurence Orchard
    ,
  • Ken I. Mills
  • University of Rostock
    ,
  • University of Hull
    ,
  • University Hospital Southampton NHS Foundation Trust
    ,
  • Queen's University Belfast
    ,
  • University College Cork
Research Output: Contribution to journal Article Peer-review

Open access

Sustainable Development Goals

  • SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well

Abstract

A Crohn's-like lymphoid reaction (CLR) is observed in about 15% of colorectal cancer (CRC) patients and is associated with favourable outcomes. To identify the immune targets recognised by CRC CLR patient sera, we immunoscreened a testes cDNA library with sera from three patients. Immunoscreening of the 18 antigens identified by SEREX with sera from normal donors showed that only the heavy chain of IgG3 (IGHG3) and a novel antigen we named UOB-COL-7, were solely recognised by sera from CRC CLR patients. ELISA showed an elevation in IgG3 levels in patients with CRC (p = 0.01). To extend our studies we analysed the expression of our SEREX-identified antigens using the RNA-sequencing dataset (GSE5206). We found that the transcript levels of multiple IGHG probesets were highly significant (p < 0.001) in their association with clinical features of CRC while above median levels of DAPK1 (p = 0.005) and below median levels of GTF2H5 (p = 0.004) and SH3RF2 (p = 0.02) were associated with improved overall survival. Our findings demonstrate the potential of SEREX-identified CRC CLR antigens to act as biomarkers for CRC and provide a rationale for their further characterization and validation.

Publication Information

Output type

Research Output: Contribution to journal Article Peer-review

Original language

English

Article number

1058

Journal (Volume, Issue Number)

Biomolecules (Volume 12, Issue 8)

Publication milestones

  • Accepted/In press - 25/07/2022
  • Published - 29/07/2022

Publication status

Published - 29/07/2022

External Publication IDs

  • handle.net: 10547/625526
  • Scopus: 85137126699
  • PubMed: 36008952

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