Skip to search boxSkip to navigationSkip to main content

Expression and subcellular localization of multifunctional calmodulin-dependent protein kinases-I, -II and -IV are altered in rat hippocampal CA1 neurons after induction of long-term potentiation

  • Min Tak
    ,
  • Bushra Ahmed
    ,
  • Fuminori Yamaguchi
    ,
  • Toyohiro Tsumura
    ,
  • Takaya Gotoh
    ,
  • Katsuyoshi Sugimoto
Research Output: Contribution to journal Article Peer-review

Abstract

Long-term potentiation (LTP) is considered to be associated with an increase in expression as well as activity of Ca2+/calmodulin-dependent protein kinases (CaMKs). LTP-induced and control hippocampal slices were studied by immunohistochemical and electronmicroscopic analyses using anti-CaMK-I, -II and -IV antibodies. All three kinases were demonstrated to increase their expression in CA1 neurons. CaMK-I was shown to mainly localize in the cytoplasm of the control and LTP-induced neurons, and a significant increase of immunoreactivity was observed in the latter neurons. A part of CaMK-I was found to translocate to the nuclei of LTP-induced hippocampal CA1 neurons. Direct evidence of the translocation of CaMK-II from cytoplasm to nuclei in LTP was demonstrated by immuno-electronmicroscopy. A significant increase in expression of CaMK-IV in the nuclei was also observed. Our data suggest that all the three CaMKs were actively involved in nuclear Ca2+- signaling in LTP.

Publication Information

Output type

Research Output: Contribution to journal Article Peer-review

Original language

English

Pages from-to (Number of pages)

Pages 149-153

Journal (Volume, Issue Number)

Neuroscience Letters (Volume 290, Issue 2)

Publication milestones

  • Published - 01/01/2000

Publication status

Published - 01/01/2000

ISSN

0304-3940

External Publication IDs

  • handle.net: 10547/302091
  • Scopus: 0034714485

Access to documents

Publication metrics