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Encapsulation of α-tocopherol in whey protein isolate/chitosan particles using oil-in-water emulsion with optimal stability and bioaccessibility

  • Weili Xu
    ,
  • Kangxing Lv
    ,
  • Wei Mu
    ,
  • Shaobo Zhou
    ,
  • Yang Yang
  • Harbin Institute of Technology
Research Output: Contribution to journal Article Peer-review

Open access

Abstract

The aim of this study was to develop an oil-in-water (O/W) emulsion using whey protein isolate (WPI)-chitosan (CN) complex to encapsulate α-tocopherol and to characterize their stability and bioaccessibility in vitro. The O/W emulsions prepared under the optimal conditions (mass ratio of WPI:CN = 1: 1, corn oil containing 5 g/100 g of α-tocopherol) exhibited a monomodal distribution (d = 803.3 ± 6.9 nm) with encapsulation rate of 86.3 ± 2.3%. The emulsions were stable under NaCl (0–150 mmol/L), sugar (0–5 g/100 g), 55 °C for 30 min, pH 5–6.5, even storage for 20 d at 4 °C and 25 °C. During gastric digestion, WPI situated at the surface of emulsion particles can be digested into small molecular peptides by pepsin, but the structure of the core-shell particles remained due to the cross-linking with CN. During intestinal digestion, the structure of the particles disintegrated over the digestion time, and the inner-oil phase was released. Release profiles of the α-tocopherol and free fatty acids showed a burst effect followed by slow release. These results suggest that the WPI-CN complex could be used to achieve a controlled and sustainable release of liposoluble bioactive compounds from O/W emulsions.

Publication Information

Output type

Research Output: Contribution to journal Article Peer-review

Original language

English

Article number

111724

Pages from-to (Number of pages)

Pages 111724

Journal (Volume, Issue Number)

LWT (Volume 148, Issue 148)

Publication milestones

  • Accepted/In press - 13/05/2021
  • Published - 23/05/2021

Publication status

Published - 23/05/2021

ISSN

0023-6438

External Publication IDs

  • handle.net: 10547/624996
  • Scopus: 85106912586

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