Elucidation of xenobiotic metabolism pathways in human skin and human skin models by proteomic profiling

Zheying Zhu
,
Sven van Eijl
,
John Cupitt
,
Magdalena Gierula
,
Christine Götz
,
Ellen Fritsche

Imperial College London
,
Heinrich Heine University Düsseldorf
,
RWTH Aachen University

Research Output: Contribution to journal Article Peer-review

Open access

Abstract

Human skin has the capacity to metabolise foreign chemicals (xenobiotics), but knowledge of the various enzymes involved is incomplete. A broad-based unbiased proteomics approach was used to describe the profile of xenobiotic metabolising enzymes present in human skin and hence indicate principal routes of metabolism of xenobiotic compounds. Several in vitro models of human skin have been developed for the purpose of safety assessment of chemicals. The suitability of these epidermal models for studies involving biotransformation was assessed by comparing their profiles of xenobiotic metabolising enzymes with those of human skin.