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Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors

  • Bushra Ahmed
    ,
  • Sridhara Chakravarthy
    ,
  • Ruben Eggers
    ,
  • Wim TJMC Hermens
    ,
  • Jing Zhang
    ,
  • Simone P. Niclou
  • Netherlands Institute for Neuroscience
    ,
  • University of Nottingham
    ,
  • University College London
Research Output: Contribution to journal Article Peer-review

Open access

Abstract

Inactivating genes in vivo is an important technique for establishing their function in the adult nervous system. Unfortunately, conventional knockout mice may suffer from several limitations including embryonic or perinatal lethality and the compensatory regulation of other genes. One approach to producing conditional activation or inactivation of genes involves the use of Cre recombinase to remove loxP-flanked segments of DNA. We have studied the effects of delivering Cre to the hippocampus and neocortex of adult mice by injecting replication-deficient adeno-associated virus (AAV) and lentiviral (LV) vectors into discrete regions of the forebrain

Publication Information

Output type

Research Output: Contribution to journal Article Peer-review

Original language

English

Pages from-to (Number of pages)

Pages 4-13

Journal (Volume, Issue Number)

BMC Neuroscience (Volume 5, Issue 1)

Publication milestones

  • Published - 30/01/2004

Publication status

Published - 30/01/2004

ISSN

1471-2202

External Publication IDs

  • handle.net: 10547/302088
  • Scopus: 2642570238

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