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Effects of AC/DC electric fields on stretching DNA molecules

  • Ying Wang
    ,
  • Mingyan Gao
    ,
  • Yingmin Qu
    ,
  • Jun Hu
    ,
  • Ying Xie
    ,
  • Ziyu Liu
  • Changchun University of Science and Technology
Research Output: Contribution to journal Article Peer-review

Abstract

The effects of AC/DC electric fields on stretching DNA molecules were discussed in this work. In the experiments of stretching DNA molecules with AC/DC electric fields, the voltage range was changed from 0V to 10V, and the frequency of AC electric field was kept at 50kHz. An atomic force microscope (AFM) was used to obtain DNA distributions under different electric fields. DNA molecules were curved and randomly distributed in solution if there was not any force applied to them. When an AC electric field was applied to the DNA sample, the curvature of DNA molecules was decreased gradually, and the stretching result was more obvious with the increase of voltage from 0.1V to 5V. The DNA molecules were broken when the voltage was increased to 6V. However, under the DC electric field, the stretching result of DNA molecules reached to their optimum state when the voltage was 2V, and they kept their steady state even though larger electric field intensities applied to the electrodes. The results can be used for the study of DNA-DNA, protein-DNA and quantum dot-DNA interactions and for the exploration of DNA biophysical properties.

Publication Information

Output type

Research Output: Contribution to journal Article Peer-review

Original language

English

Pages from-to (Number of pages)

Pages 2050065

Journal (Volume, Issue Number)

Nano (Volume 15, Issue 5)

Publication milestones

  • Published - 01/05/2020

Publication status

Published - 01/05/2020

ISSN

1793-2920

External Publication IDs

  • handle.net: 10547/624394
  • Scopus: 85085360581

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