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Effect of culture medium on morphogenic processes in vitro in Cinchona officinalis L.

  • José Antonio Moreno Serrano
    ,
  • César Pérez Ruíz
    ,
  • Ivonne Moreno Fierro
    ,
  • Jorge Moreno-Fierro
  • Technical University of Madrid
Research Output: Contribution to journal Article Peer-review

Open access

Abstract

This paper describes the effect of the various plant growth regulators of the culture medium on morphogenic processes in vitro in Cinchona officinalis L, a highly vulnerable species from southern Ecuador. To do this, different concentrations of NaOCl were used in combination with different immersion times for seed disinfection; for seed germination in vitro GA3 was added to the MS basal culture medium in different concentrations, and for morphogenic processes in vitro, different concentrations of auxins and cytokinins were combined. The decrease in the contamination rate was with high concentrations of NaOCl and an increase in the germination rate in 45 days with the addition of 1.0 mg L-1 GA3 to the culture medium the hormonal combination of 0.5 mg L-1 NAA + 2.5 mg L-1 BAP showed a high rate of shoot proliferation and with 1.0 mg L-1 NAA a high number of roots was obtained. In the callogenesis phase, the best results were obtained with 1.0 mg L-1 2,4-D + 0.5 mg L-1 BAP for callus proliferation. In vitro propagation protocols were generated in Cinchona officinalis L, for the preservation and conservation of the species.

Publication Information

Output type

Research Output: Contribution to journal Article Peer-review

Original language

English

Pages from-to (Number of pages)

Pages 55-68

Journal (Volume, Issue Number)

Revista de la Facultad de Ciencias Agrarias (Volume 51, Issue 1)

Publication milestones

  • Published - 30/06/2019

Publication status

Published - 30/06/2019

ISSN

0370-4661

External Publication IDs

  • handle.net: 10547/624137
  • Scopus: 85068520668

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