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Downhill running and exercise in hot environments increase leukocyte Hsp72 (HSPA1A) and Hsp90α (HSPC1) gene transcripts

  • James A. Tuttle
    ,
  • Paul C. Castle
    ,
  • Alan J. Metcalfe
    ,
  • Adrian W. Midgley
    ,
  • Lee Taylor
    ,
  • Mark P. Lewis
  • Edith Cowan University
    ,
  • Edge Hill University
    ,
  • Loughborough University
Research Output: Other contribution Other contribution Peer-review

Open access

Abstract

Stressors within humans and other species activate Hsp72 and Hsp90 mRNA transcription, although it is unclear which environmental temperature or treadmill gradient induces the largest increase. To determine the optimal stressor for priming the Hsp system, physically active but not heat-acclimated participants (19.8 1.9 and 20.9 3.6 yr) exercised at lactate threshold in either temperate (20°C, 50% relative humidity; RH) or hot (30°C, 50% RH) environmental conditions. Within each condition, participants completed a flat running (temperate flat or hot flat) and a downhill running (temperate downhill or hot downhill) experimental trial in a randomized counterbalanced order separated by at least 7 days. Venous blood samples were taken immediately before (basal), immediately after exercise, and 3 and 24 h postexercise. RNA was extracted from leukocytes and RT-quantitative PCR conducted to determine Hsp72 and Hsp90 mRNA relative expression. Leukocyte Hsp72 mRNA was increased immediately after exercise following downhill running (1.9 0.9-fold) compared with flat running (1.3 0.4-fold; P 0.001) and in hot (1.9 0.6-fold) compared with temperate conditions (1.1 0.5-fold; P 0.003). Leukocyte Hsp90 mRNA increased immediately after exercise following downhill running (1.4 0.8-fold) compared with flat running (0.9 0.6-fold; P 0.002) and in hot (1.6 1.0-fold) compared with temperate conditions (0.9 0.6-fold; P 0.003). Downhill running and exercise in hot conditions induced the largest stimuli for leukocyte Hsp72 and Hsp90 mRNA increases.

Publication Information

Output type

Research Output: Other contribution Other contribution Peer-review

Original language

English

Publication milestones

  • Accepted/In press - 18/02/2015
  • Published - 15/04/2015

Publication status

Published - 15/04/2015

Volume

118

Publisher

American Physiological Society, United States

External Publication IDs

  • handle.net: 10547/624719
  • Scopus: 84928349651

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