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Direct imaging of antigen-antibody binding by atomic force microscopy

  • Jing Hu
    ,
  • Mingyan Gao
    ,
  • Zuobin Wang
    ,
  • Yujuan Chen
    ,
  • Zhengxun Song
    ,
  • Hongmei Xu
  • Changchun University of Science and Technology
Research Output: Contribution to journal Article Peer-review

Sustainable Development Goals

  • SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well

Abstract

Direct observation of antigen-antibody binding at the nanoscale has always been a considerable challenging problem, and researchers have made tremendous efforts on it. In this study, the morphology of biotinylated antibody-specific Immunoglobulin E (IgE) immune complexes has been successfully imaged by atomic force microscopy (AFM) in the tapping-mode. The AFM images indicated that the individual immune complex was composed of an IgE and a biotinylated antibody. Excitingly, it is the first time that we have actually seen the IgE binding to biotinylated antibody. Alternatively, information on the length of IgE, biotinylated antibodies and biotinylated antibody-specific IgE immune complexes were also obtained, respectively. These results indicate the versatility of AFM technology in the identification of antigen-antibody binding. This work not only lays the basis for the direct imaging of the biotinylated antibody-IgE by AFM, but also offers valuable information for studying the targeted therapy and vaccine development in the future.

Publication Information

Output type

Research Output: Contribution to journal Article Peer-review

Original language

English

Pages from-to (Number of pages)

Pages 293-300

Journal (Volume, Issue Number)

Applied Nanoscience (Switzerland) (Volume 11)

Publication milestones

  • Accepted/In press - 15/09/2020
  • Published - 24/09/2020

Publication status

Published - 24/09/2020

ISSN

2190-5517

External Publication IDs

  • handle.net: 10547/624563
  • Scopus: 85091463389

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