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Controlled manipulation of TRAIL into single human colon cancer cells using atomic force microscope

  • Yingmin Qu
    ,
  • Jinyun Liu
    ,
  • Guoliang Wang
    ,
  • Zhengxun Song
    ,
  • Zuobin Wang
  • Changchun University of Science and Technology
Research Output: Chapter in Book/Report/Conference proceeding Conference contribution Peer-review

Sustainable Development Goals

  • SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well

Abstract

In this study, an AFM tip was used to penetrate the human colon cancer cells (SW480) in the culture medium containing pEGFP-N1-TRAIL plasmids. The trail plasmids encoded with the enhanced green fluorescent protein (EGFP) were moved into the SW480 cells through membrane holes created by the AFM probe. Following the penetration, the culture medium was changed into the RPMI1640 medium supplemented with 10% of fetal bovine serum and incubated for 24h. The expression of PEGFP-N1-TRAIL in SW480 cells was then observed by inverted fluorescence microscope. The experiment results indicate that the AFM tip can be used to penetrate the membranes of targeted cells individually.

Publication Information

Output type

Research Output: Chapter in Book/Report/Conference proceeding Conference contribution Peer-review

Original language

English

Pages from-to (Number of pages)

Pages 345-348

Publication milestones

  • Published - 08/02/2018

Publication status

Published - 08/02/2018

Publisher

Institute of Electrical and Electronics Engineers Inc., United States

ISBN (Print)

9781538610817

External Publication IDs

  • handle.net: 10547/623872

Host publication title

2017 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO)

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