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Conservation of inner nuclear membrane targeting sequences in mammalian Pom121 and yeast Heh2 membrane proteins

  • Noorjahan Basheer
    ,
  • Annemarie Kralt
    ,
  • Vincent Van Den Boom
    ,
  • Ravi K. Lokareddy
    ,
  • Anton Steen
    ,
  • Gino Cingolani
Research Output: Contribution to journal Article Peer-review

Open access

Abstract

Endoplasmic reticulum-synthesized membrane proteins traffic through the nuclear pore complex (NPC) en route to the inner nuclear membrane (INM). Although many membrane proteins pass the NPC by simple diffusion, two yeast proteins, ScSrc1/ScHeh1 and ScHeh2, are actively imported. In these proteins, a nuclear localization signal (NLS) and an intrinsically disordered linker encode the sorting signal for recruiting the transport factors for FG-Nup and RanGTP-dependent transport through the NPC. Here we address whether a similar import mechanism applies in metazoans. We show that the (putative) NLSs of metazoan HsSun2, MmLem2, HsLBR, and HsLap2β are not sufficient to drive nuclear accumulation of a membrane protein in yeast, but the NLS from RnPom121 is. This NLS of Pom121 adapts a similar fold as the NLS of Heh2 when transport factor bound and rescues the subcellular localization and synthetic sickness of Heh2ΔNLS mutants. Consistent with the conservation of these NLSs, the NLS and linker of Heh2 support INM localization in HEK293T cells. The conserved features of the NLSs of ScHeh1, ScHeh2, and RnPom121 and the effective sorting of Heh2-derived reporters in human cells suggest that active import is conserved but confined to a small subset of INM proteins.

Publication Information

Output type

Research Output: Contribution to journal Article Peer-review

Original language

English

Pages from-to (Number of pages)

Pages 3301-3312

Journal (Volume, Issue Number)

Molecular Biology of the Cell (Volume 26, Issue 18)

Publication milestones

  • Accepted/In press - 08/07/2015
  • Published - 15/07/2015

Publication status

Published - 15/07/2015

ISSN

1059-1524

External Publication IDs

  • handle.net: 10547/624675
  • Scopus: 84942103985

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