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Complete sequence of the gene encoding the bacterioferritin subunit of Mycobacterium avium subspecies silvaticum

  • Arthur Hosie
    ,
  • Neil F. Inglis
    ,
  • Karen Stevenson
    ,
  • J.Michael Sharp
  • Moredun Research Institute
Research Output: Contribution to journal Article Peer-review

Abstract

A gene encoding the bacterioferritin subunit (Bfr) of Mycobacterium avium (Ma) subspecies silvaticum has been cloned, sequenced and expressed. The 477-bp open reading frame codes for 159 amino acids, which were shown to share up to 92% identity with the Bfr of five bacterial genera. The recombinant Bfr exhibits serological cross-reactivity with Ma paratuberculosis antigen D, a protein of approx. 20 kDa in cell lysates of Ma paratuberculosis and Ma silvaticum and a protein of 20-22 kDa in sonicates of M. leprae.

Publication Information

Output type

Research Output: Contribution to journal Article Peer-review

Original language

English

Pages from-to (Number of pages)

Pages 205-206

Journal (Volume, Issue Number)

Gene (Volume 150, Issue 1)

Publication milestones

  • Published - 01/01/1994

Publication status

Published - 01/01/1994

ISSN

0378-1119

External Publication IDs

  • handle.net: 10547/293972
  • Scopus: 0028099875

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