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Characterization of the spore surface and exosporium proteins of Clostridium sporogenes; implications for Clostridium botulinum group I strains.

  • Thamarai Janganan
    ,
  • Nic Mullin
    ,
  • Svetomir B. Tzokov
    ,
  • Sandra Stringer
    ,
  • Robert P. Fagan
    ,
  • Jamie K. Hobbs
  • University of Sheffield
    ,
  • Norwich Research Park
Research Output: Contribution to journal Article Peer-review

Open access

Abstract

Clostridium sporogenes is a non-pathogenic close relative and surrogate for Group I (proteolytic) neurotoxin-producing Clostridium botulinum strains. The exosporium, the sac-like outermost layer of spores of these species, is likely to contribute to adhesion, dissemination, and virulence. A paracrystalline array, hairy nap, and several appendages were detected in the exosporium of C. sporogenes strain NCIMB 701792 by EM and AFM. The protein composition of purified exosporium was explored by LC-MS/MS of tryptic peptides from major individual SDS-PAGE-separated protein bands, and from bulk exosporium. Two high molecular weight protein bands both contained the same protein with a collagen-like repeat domain, the probable constituent of the hairy nap, as well as cysteine-rich proteins CsxA and CsxB. A third cysteine-rich protein (CsxC) was also identified. These three proteins are also encoded in C. botulinum Prevot 594, and homologues (75-100% amino acid identity) are encoded in many other Group I strains. This work provides the first insight into the likely composition and organization of the exosporium of Group I C. botulinum spores.

Publication Information

Output type

Research Output: Contribution to journal Article Peer-review

Original language

English

Pages from-to (Number of pages)

Pages 205-12

Journal (Volume, Issue Number)

Food Microbiology (Volume 59)

Publication milestones

  • Published - 06/06/2016

Publication status

Published - 06/06/2016

ISSN

0740-0020

External Publication IDs

  • handle.net: 10547/622746
  • Scopus: 84975493976

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