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Biochemical and histological changes associated with downy mildew (Plasmopara halstedii (Farl) Berl. and de Toni) infection in sunflower (Helianthus annuus L.)

  • B.R. Prashanth Kumar
    ,
  • Mahesh J. Kulkarni
    ,
  • B.N. Veena Rao
    ,
  • K. Chandrika
    ,
  • V.R. Balakrishna Gowda
    ,
  • D. Theertha Prasad
Research Output: Contribution to journal Article Peer-review

Open access

Abstract

Biochemical changes associated with downy mildew infection in sunflower revealed an increase in the total soluble proteins, 0-40% ammonium sulfate fractionated proteins and total soluble sugars. Molecular sieve chromatography of 0-40% ammonium sulphate fraction revealed for presence of a high molecular weight protein and polysaccharide in the downy mildew infected sunflower leaves. Antibodies raised against the high molecular weight protein and polysaccharide were used in the western blot/dot blot analysis. It has been shown that the downy mildew disease also induces PR proteins, which have antigenic homology with PR-S protein, a member of the PR-5 class of proteins. An increase in iPA (isopentenyl adenosine) content in the infected sample was observed. A positive correlation exists between iPA level, 0-40% protein and total sugar content. Histological studies revealed that the downy mildew fungal mycelium extensively proliferates, ramifies extensively the leaf tissue and forms a nutritional link with the host cell by producing the intracellular haustorium.

Publication Information

Output type

Research Output: Contribution to journal Article Peer-review

Original language

English

Pages from-to (Number of pages)

Pages 1-18

Journal (Volume, Issue Number)

Helia (Volume 23, Issue 33)

Publication milestones

  • Accepted/In press - 07/09/2000
  • Published - 01/12/2000

Publication status

Published - 01/12/2000

ISSN

1018-1806

External Publication IDs

  • Scopus: 85026074360

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