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ATP-binding cassette transporter VcaM from Vibrio cholerae is dependent on the outer membrane factor family for Its function

  • Wen-Jung Lu
    ,
  • Hsuan-Ju Lin
    ,
  • Thamarai Janganan
    ,
  • Cheng-Yu Li
    ,
  • Wen-Chiang Chin
    ,
  • Vassiliy N Bavro
Research Output: Contribution to journal Article Peer-review

Open access

Abstract

strains lacking the principal secondary transporter AcrB (Acriflavine resistance protein), as well as cells lacking the outer membrane factor (OMF) TolC (Tolerance to colicins). Consistent with the ATPase function of VcaM we found it to be susceptible to sodium orthovanadate (NaOV), however, we also found a clear dependency of VcaM function on TolC. Inhibitors targeting secondary active transporters had no effects on either VcaM-conferred resistance or Hoechst 33342 accumulation, suggesting that VcaM might be capable of engaging with the TolC-channel without periplasmic mediation by additional transporters. Our findings are indicative of VcaM being capable of a one-step substrate translocation from cytosol to extracellular space utilising the TolC-channel, making it the only multidrug ABC-transporter outside of the MacB-family with demonstrable TolC-dependency.

Publication Information

Output type

Research Output: Contribution to journal Article Peer-review

Original language

English

Journal (Volume, Issue Number)

International Journal of Molecular Sciences (Volume 19, Issue 4)

Publication milestones

  • Published - 27/03/2018

Publication status

Published - 27/03/2018

ISSN

1661-6596

External Publication IDs

  • handle.net: 10547/622747
  • Scopus: 85044622842

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