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Atomic force microscopy imaging of the G-banding process of chromosomes

  • Bowei Wang
    ,
  • Jiani Li
    ,
  • Jianjun Dong
    ,
  • Fan Yang
    ,
  • Kaige Qu
    ,
  • Ying Wang
  • Changchun University of Science and Technology
    ,
  • Changli Nano Biotechnology
Research Output: Contribution to journal Article Peer-review

Open access

Abstract

The chromosome is an important genetic material carrier in living individuals and the spatial conformation (mainly referring to the chromosomal structure, quantity, centromere position and other morphological information) may be abnormal or mutated. Thus, it may generate a high possibility to cause diseases. Generally, the karyotype of chromosome G-bands is detected and analyzed using an optical microscope. However, it is difficult to detect the G-band structures for traditional optical microscopes on the nanometer scale. Herein, we have studied the detection method of chromosome G-band samples by atomic force microscopy (AFM) imaging. The structures of chromosome G-banding are studied with different trypsin treatment durations. The experiment result shows that the treatment duration of 20 s is the best time to form G-band structures. The AFM images show the structures of chromosome G-bands which cannot be observed under an optical microscope. This work provides a new way for the detection and diagnosis of chromosome diseases on the nanometer scale.

Publication Information

Output type

Research Output: Contribution to journal Article Peer-review

Original language

English

Pages from-to (Number of pages)

Pages 249-255

Journal (Volume, Issue Number)

Applied Nanoscience (Switzerland) (Volume 11)

Publication milestones

  • Accepted/In press - 13/10/2020
  • Published - 24/10/2020

Publication status

Published - 24/10/2020

ISSN

2190-5509

External Publication IDs

  • handle.net: 10547/624777
  • Scopus: 85093971313

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