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Analysis of colchicine-induced effects on hepatoma and hepatpcyte cells by atomic force microscopy

  • Lanjiao Liu
    ,
  • Zuobin Wang
    ,
  • Wenxiao Zhang
    ,
  • Xinyao Zhu
    ,
  • Li Li
    ,
  • Zhankun Weng
Research Output: Contribution to journal Article Peer-review

Sustainable Development Goals

  • SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well

Abstract

Biomechanical properties of cells are altered by many diseases. Cancer cell metastasis is related to the properties such as the cell stiffness that influences cell proliferation, differentiation and migration. In this paper, we used an atomic force microscope to analyze the colchicine-induced effects on the mechanical properties of hepatocyte (HL-7702 cells) and hepatoma cells (SMCC-7721 cells) in culture at the nanoscale. The cells were exposed to a solution with a normal dose of colchicine for two, four and six hours. Surface topographic images showed that colchicine decreased the stability of the cytoskeleton. After the same six-hour treatment in a solution with a normal dose of colchicine, the biomechanical properties of HL-7702 cells were almost unchanged. However, the stiffness and the adhesion force of the SMCC-7721 cells were clearly increased (more than twofold of the normal values), especially after four hours. The deformability of SMCC-7721 cancer cells was significantly decreased within the six-hour treatment in the solution with a normal dose of colchicine. Analysis of the biomechanical properties of post-treatment hepatoma cells provided a complementary explanation for the mechanism of action of colchicine on cells at the nanoscale. This method is expected to allow the monitoring of potential metastatic cancer cell changes, thus preventing the emergence and the transmission of disease, and improving the diagnosis of cancer.

Publication Information

Output type

Research Output: Contribution to journal Article Peer-review

Original language

English

Pages from-to (Number of pages)

Pages 4248-4254

Journal (Volume, Issue Number)

Journal of Nanoscience and Nanotechnology (Volume 18, Issue 6)

Publication milestones

  • Accepted/In press - 01/06/2018
  • Published - 01/06/2018

Publication status

Published - 01/06/2018

ISSN

1533-4880

External Publication IDs

  • handle.net: 10547/623568

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