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A study on the effects of tumor-derived exosomes on hepatoma cells and hepatocytes by atomic force microscopy

  • Tuoyu Ju
    ,
  • Shuwei Wang
    ,
  • Jiajia Wang
    ,
  • Fan Yang
    ,
  • Zhengxun Song
    ,
  • Hongmei Xu
  • Changchun University of Science and Technology
    ,
  • The First Hospital of Jilin University
Research Output:
Contribution to journal
Article
 Peer-review

Sustainable Development Goals

  • SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well

Abstract

Tumor-derived exosomes (exos) are closely related to the occurrence, development and treatment of tumors. However, it is not clear how the exosomes affect the physical properties, which lead to the deterioration of the target cells. In this paper, atomic force microscopy (AFM) was used to study the effects of exosomes in HCC-LM3 cells and other cells (SMMC-7721 and HL-7702). The results showed that the HCC-LM3-exos (the exosomes secreted by HCC-LM3 cells, 50 μg mL-1) significantly promoted the proliferation and migration of HCC-LM3 cells. HCC-LM3-exos also promoted the proliferation and migration of SMMC-7721 and HL-7702 cells at 1000 and 1500 μg mL-1, respectively. With an increase in time and concentration, the proliferation effect was more significant. On comparing the mechanical properties of the three types of cells (HCC-LM3, SMMC-7721 and HL-7702 cells), the degradation degree and migration ability of the cells were from high to low in the above order. In turn, the surface roughness of the cells decreased, and adhesion and elastic modulus increased. With an increase in treatment time, surface roughness increased, while adhesion and elastic modulus decreased. These suggested that the HCC-LM3-exos could change the mechanical properties of cells, leading to their deterioration, and enhance their migration and invasion ability. In this paper, the effects of exosomes were analyzed from the perspective of the physical parameters of cells, which provide a new idea to study cancer metastasis and prognosis.

Publication Information

Output type

Research Output:
Contribution to journal
Article
 Peer-review

Original language

English

Pages from-to (Number of pages)

Pages 5458-5467

Journal (Volume, Issue Number)

Analytical Methods (Volume 12, Issue 45)

Publication milestones

  • Published - 07/12/2020

Publication status

Published - 07/12/2020

ISSN

1759-9660

Publication IDs

  • handle.net: 10547/624732
  • Scopus: 85096946797

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