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A novel approach to oral iron delivery using ferrous sulphate loaded solid lipid nanoparticles

  • Mohammed Gulrez Zariwala
    ,
  • Naba Elsaid
    ,
  • Timothy L. Jackson
    ,
  • Francisco Corral López
    ,
  • Sebastien Farnaud
    ,
  • Satyanarayana Somavarapu
  • University College London
    ,
  • King's College Hospital
    ,
  • University of Westminster
Research Output:
Contribution to journal
Article
 Peer-review

Abstract

Iron (Fe) loaded solid lipid nanoparticles (SLN's) were formulated using stearic acid and iron absorption was evaluated in vitro using the cell line Caco-2 with intracellular ferritin formation as a marker of iron absorption. Iron loading was optimised at 1% Fe (w/w) lipid since an inverse relation was observed between initial iron concentration and SLN iron incorporation efficiency. Chitosan (Chi) was included to prepare chitosan coated SLN's. Particle size analysis revealed a sub-micron size range (300.3±31.75 nm to 495.1±80.42 nm), with chitosan containing particles having the largest dimensions. As expected, chitosan (0.1%, 0.2% and 0.4% w/v) conferred a net positive charge on the particle surface in a concentration dependent manner. For iron absorption experiments equal doses of Fe (20 μM) from selected formulations (SLN-FeA and SLN-Fe-ChiB) were added to Caco-2 cells and intracellular ferritin protein concentrations determined. Caco-2 iron absorption from SLN-FeA (583.98±40.83 ng/mg cell protein) and chitosan containing SLN-Fe-ChiB (642.77±29.37 ng/mg cell protein) were 13.42% and 24.9% greater than that from ferrous sulphate (FeSO4) reference (514.66±20.43 ng/mg cell protein) (p≤0.05). We demonstrate for the first time preparation, characterisation and superior iron absorption in vitro from SLN's, suggesting the potential of these formulations as a novel system for oral iron delivery.

Publication Information

Output type

Research Output:
Contribution to journal
Article
 Peer-review

Original language

English

Pages from-to (Number of pages)

Pages 400-7

Journal (Volume, Issue Number)

International Journal of Pharmaceutics (Volume 456, Issue 2)

Publication milestones

  • Published - 18/11/2013

Publication status

Published - 18/11/2013

ISSN

0378-5173

Publication IDs

  • handle.net: 10547/623165
  • Scopus: 84886726407

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