Chronic probiotic supplementation and its effects on eHsp72 and LPS concentration following a desert-based ultramarathon

Abstract

This study investigated the effect of 12wk probiotic supplementation on the Lipopolysaccharides (LPS) and extracellular-heat-shock-protein 72 (eHsp72) response to a 7d ultra-endurance event (249.4km) in extreme heat [(average temperature ~38°C) Marathon des Sables (MDS) 2015]. Thirty-two (6 female) competitors were randomly allocated to receive probiotic, probiotic + glutamine, or no supplementation for 12wk prior to the MDS. Blood samples were collected on two occasions prior to the race [12wk (baseline) and 7d pre-race], and two further occasions post-race (6-8h and 7d post-race). Plasma eHsp72 and LPS concentrations were determined using ELISAs; V O was recorded at baseline and pre-race. A significant increase in overall mean V O was observed from baseline to pre-race (p0.05). Overall mean post-race eHsp72 concentration was significantly increased (p0.05). There was no significant change in LPS concentration from baseline to post-race in all groups (p>0.05), no difference in LPS concentration was observed between groups at any time (p>0.05). This study indicates an ineffective role of PRO and PGLn supplementation on LPS translocation and eHsp72 response to ultramarathon performance in extreme heat.

Date of Award	Dec 2015
Original language	English
Awarding Institution	University of Bedfordshire
Supervisor	Lee Taylor (Supervisor) & John Hough (Second supervisor)