Improved DNA straightening and attachment via optimal Mg2+ ionic bonding under electric field for AFM imaging in liquid phase

Ziya Liu; Hongmei Xu; Ying Wang; Fan Yang; Yaoting Yin; Sheng Zhang; Zhankun Weng; Zhengxun Song; Zuobin Wang

doi:10.1016/j.micron.2019.04.006

Improved DNA straightening and attachment via optimal Mg2+ ionic bonding under electric field for AFM imaging in liquid phase

Ziya Liu
, Hongmei Xu
, Ying Wang
, Fan Yang
, Yaoting Yin
, Sheng Zhang
, Zhankun Weng
, Zhengxun Song
, Zuobin Wang

Changchun University of Science and Technology

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

In this research, a novel method is proposed to improve DNA straightening under an applied electric field to facilitate imaging in a liquid phase by modifying the substrate with varying Mg2+ ion concentrations. A two-dimensional network of DNA structures was successfully stretched on Mg2+-modified mica substrates under a DC electric field (1 V, 1 A) and imaged in gaseous and aqueous phases by atomic force microscopy. The results revealed that an optimum concentration of Mg2+ ion (4.17 μmol/ml) allowed DNA straightening under an electric field, thus facilitating its imaging in the liquid phase. Furthermore, DNA adhesion under different concentrations of Mg2+ was measured and a maximum adhesion force of 76.19 pN was achieved. This vital work has great potential in gene knockout and targeted gene editing.

Original language	English
Pages (from-to)	102678
Journal	Micron
Volume	124
DOIs	https://doi.org/10.1016/j.micron.2019.04.006
Publication status	Published - 25 May 2019

Keywords

AFM imaging
DNA straightening
Electric field
Ion modification
Liquid phase

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10.1016/j.micron.2019.04.006

https://www.sciencedirect.com/science/article/pii/S096843281930068X

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title = "Improved DNA straightening and attachment via optimal Mg2+ ionic bonding under electric field for AFM imaging in liquid phase",

abstract = "In this research, a novel method is proposed to improve DNA straightening under an applied electric field to facilitate imaging in a liquid phase by modifying the substrate with varying Mg2+ ion concentrations. A two-dimensional network of DNA structures was successfully stretched on Mg2+-modified mica substrates under a DC electric field (1 V, 1 A) and imaged in gaseous and aqueous phases by atomic force microscopy. The results revealed that an optimum concentration of Mg2+ ion (4.17 μmol/ml) allowed DNA straightening under an electric field, thus facilitating its imaging in the liquid phase. Furthermore, DNA adhesion under different concentrations of Mg2+ was measured and a maximum adhesion force of 76.19 pN was achieved. This vital work has great potential in gene knockout and targeted gene editing.",

keywords = "AFM imaging, DNA straightening, Electric field, Ion modification, Liquid phase",

author = "Ziya Liu and Hongmei Xu and Ying Wang and Fan Yang and Yaoting Yin and Sheng Zhang and Zhankun Weng and Zhengxun Song and Zuobin Wang",

year = "2019",

month = may,

day = "25",

doi = "10.1016/j.micron.2019.04.006",

language = "English",

volume = "124",

pages = "102678",

journal = "Micron",

issn = "0968-4328",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Improved DNA straightening and attachment via optimal Mg2+ ionic bonding under electric field for AFM imaging in liquid phase

AU - Liu, Ziya

AU - Xu, Hongmei

AU - Wang, Ying

AU - Yang, Fan

AU - Yin, Yaoting

AU - Zhang, Sheng

AU - Weng, Zhankun

AU - Song, Zhengxun

AU - Wang, Zuobin

PY - 2019/5/25

Y1 - 2019/5/25

N2 - In this research, a novel method is proposed to improve DNA straightening under an applied electric field to facilitate imaging in a liquid phase by modifying the substrate with varying Mg2+ ion concentrations. A two-dimensional network of DNA structures was successfully stretched on Mg2+-modified mica substrates under a DC electric field (1 V, 1 A) and imaged in gaseous and aqueous phases by atomic force microscopy. The results revealed that an optimum concentration of Mg2+ ion (4.17 μmol/ml) allowed DNA straightening under an electric field, thus facilitating its imaging in the liquid phase. Furthermore, DNA adhesion under different concentrations of Mg2+ was measured and a maximum adhesion force of 76.19 pN was achieved. This vital work has great potential in gene knockout and targeted gene editing.

AB - In this research, a novel method is proposed to improve DNA straightening under an applied electric field to facilitate imaging in a liquid phase by modifying the substrate with varying Mg2+ ion concentrations. A two-dimensional network of DNA structures was successfully stretched on Mg2+-modified mica substrates under a DC electric field (1 V, 1 A) and imaged in gaseous and aqueous phases by atomic force microscopy. The results revealed that an optimum concentration of Mg2+ ion (4.17 μmol/ml) allowed DNA straightening under an electric field, thus facilitating its imaging in the liquid phase. Furthermore, DNA adhesion under different concentrations of Mg2+ was measured and a maximum adhesion force of 76.19 pN was achieved. This vital work has great potential in gene knockout and targeted gene editing.

KW - AFM imaging

KW - DNA straightening

KW - Electric field

KW - Ion modification

KW - Liquid phase

U2 - 10.1016/j.micron.2019.04.006

DO - 10.1016/j.micron.2019.04.006

M3 - Article

SN - 0968-4328

VL - 124

SP - 102678

JO - Micron

JF - Micron

ER -

Improved DNA straightening and attachment via optimal Mg2+ ionic bonding under electric field for AFM imaging in liquid phase

Abstract

Keywords

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