Evaluations of 5-fluorourcil treated lung cancer cells by atomic force microscopy

Xiaolin Jiang; Ke Ma; Cuihua Hu; Mingyan Gao; Jiashuo Zhang; Ying Wang; Yujuan Chen; Zhengxun Song; Zuobin Wang

doi:10.1039/C9AY01485C

Evaluations of 5-fluorourcil treated lung cancer cells by atomic force microscopy

Xiaolin Jiang
, Ke Ma
, Cuihua Hu
, Mingyan Gao
, Jiashuo Zhang
, Ying Wang
, Yujuan Chen
, Zhengxun Song
, Zuobin Wang

Changchun University of Science and Technology

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

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Abstract

Atomic force microscopy (AFM) can be used to obtain the physical information of single live cancer cells; however, the physical changes in live cells with time based on AFM remain to be studied, which play a key role in the evaluation of the efficacy and side effects of drugs. Herein, the treatment of the A549 cell line with the anticarcinogen 5-fluorouracil has been discussed based on the AFM analysis of their continuous physical changes, including their surface morphology, height, adhesion and Young's modulus, with time. In comparison, the African green monkey kidney (Vero) cell line was tested as normal cells to determine the side effects of 5-fluorouracil. The results show that the optimal concentration of 5-fluorouracil is about 500 μM, which presents the best anticancer effect and mild side effects.

Original language	English
Pages (from-to)	4977-4982
Journal	Analytical Methods
Volume	11
DOIs	https://doi.org/10.1039/C9AY01485C
Publication status	Published - 6 Sept 2019

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

atomic force microscopy
cancer cells

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10.1039/C9AY01485C

ViewPageProof AY c9ay01485cAccepted author manuscript, 0.98 MBLicence: CC BY-NC-ND

https://pubs.rsc.org/en/content/articlelanding/2019/ay/c9ay01485c/unauth%23%21divAbstract

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title = "Evaluations of 5-fluorourcil treated lung cancer cells by atomic force microscopy",

abstract = "Atomic force microscopy (AFM) can be used to obtain the physical information of single live cancer cells; however, the physical changes in live cells with time based on AFM remain to be studied, which play a key role in the evaluation of the efficacy and side effects of drugs. Herein, the treatment of the A549 cell line with the anticarcinogen 5-fluorouracil has been discussed based on the AFM analysis of their continuous physical changes, including their surface morphology, height, adhesion and Young's modulus, with time. In comparison, the African green monkey kidney (Vero) cell line was tested as normal cells to determine the side effects of 5-fluorouracil. The results show that the optimal concentration of 5-fluorouracil is about 500 μM, which presents the best anticancer effect and mild side effects.",

keywords = "atomic force microscopy, cancer cells",

author = "Xiaolin Jiang and Ke Ma and Cuihua Hu and Mingyan Gao and Jiashuo Zhang and Ying Wang and Yujuan Chen and Zhengxun Song and Zuobin Wang",

year = "2019",

month = sep,

day = "6",

doi = "10.1039/C9AY01485C",

language = "English",

volume = "11",

pages = "4977--4982",

journal = "Analytical Methods",

issn = "1759-9660",

publisher = "Royal Society of Chemistry",

}

TY - JOUR

T1 - Evaluations of 5-fluorourcil treated lung cancer cells by atomic force microscopy

AU - Jiang, Xiaolin

AU - Ma, Ke

AU - Hu, Cuihua

AU - Gao, Mingyan

AU - Zhang, Jiashuo

AU - Wang, Ying

AU - Chen, Yujuan

AU - Song, Zhengxun

AU - Wang, Zuobin

PY - 2019/9/6

Y1 - 2019/9/6

N2 - Atomic force microscopy (AFM) can be used to obtain the physical information of single live cancer cells; however, the physical changes in live cells with time based on AFM remain to be studied, which play a key role in the evaluation of the efficacy and side effects of drugs. Herein, the treatment of the A549 cell line with the anticarcinogen 5-fluorouracil has been discussed based on the AFM analysis of their continuous physical changes, including their surface morphology, height, adhesion and Young's modulus, with time. In comparison, the African green monkey kidney (Vero) cell line was tested as normal cells to determine the side effects of 5-fluorouracil. The results show that the optimal concentration of 5-fluorouracil is about 500 μM, which presents the best anticancer effect and mild side effects.

AB - Atomic force microscopy (AFM) can be used to obtain the physical information of single live cancer cells; however, the physical changes in live cells with time based on AFM remain to be studied, which play a key role in the evaluation of the efficacy and side effects of drugs. Herein, the treatment of the A549 cell line with the anticarcinogen 5-fluorouracil has been discussed based on the AFM analysis of their continuous physical changes, including their surface morphology, height, adhesion and Young's modulus, with time. In comparison, the African green monkey kidney (Vero) cell line was tested as normal cells to determine the side effects of 5-fluorouracil. The results show that the optimal concentration of 5-fluorouracil is about 500 μM, which presents the best anticancer effect and mild side effects.

KW - atomic force microscopy

KW - cancer cells

U2 - 10.1039/C9AY01485C

DO - 10.1039/C9AY01485C

M3 - Article

SN - 1759-9660

VL - 11

SP - 4977

EP - 4982

JO - Analytical Methods

JF - Analytical Methods

ER -

Evaluations of 5-fluorourcil treated lung cancer cells by atomic force microscopy

Abstract

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