Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors

Bushra Ahmed; Sridhara Chakravarthy; Ruben Eggers; Wim TJMC Hermens; Jing Zhang; Simone P. Niclou; Christiaan Levelt; Fred Sablitzky; Patrick N. Anderson; A.R. Lieberman; Joost Verhaagen

doi:10.1186/1471-2202-5-4

Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors

Bushra Ahmed
, Sridhara Chakravarthy
, Ruben Eggers
, Wim TJMC Hermens
, Jing Zhang
, Simone P. Niclou
, Christiaan Levelt
, Fred Sablitzky
, Patrick N. Anderson
, A.R. Lieberman
, Joost Verhaagen

Institute of Biomedical and Environmental Science & Technology (IBEST)

Research output: Contribution to journal › Article › peer-review

91 Citations (Scopus)

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Abstract

Inactivating genes in vivo is an important technique for establishing their function in the adult nervous system. Unfortunately, conventional knockout mice may suffer from several limitations including embryonic or perinatal lethality and the compensatory regulation of other genes. One approach to producing conditional activation or inactivation of genes involves the use of Cre recombinase to remove loxP-flanked segments of DNA. We have studied the effects of delivering Cre to the hippocampus and neocortex of adult mice by injecting replication-deficient adeno-associated virus (AAV) and lentiviral (LV) vectors into discrete regions of the forebrain

Original language	English
Pages (from-to)	4-13
Journal	BMC Neuroscience
Volume	5
Issue number	1
DOIs	https://doi.org/10.1186/1471-2202-5-4
Publication status	Published - 30 Jan 2004

Keywords

Genetics

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10.1186/1471-2202-5-4

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https://bmcneurosci.biomedcentral.com/articles/10.1186/1471-2202-5-4

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Ahmed, B., Chakravarthy, S., Eggers, R., Hermens, W. TJMC., Zhang, J., Niclou, S. P., Levelt, C., Sablitzky, F., Anderson, P. N., Lieberman, A. R., & Verhaagen, J. (2004). Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors. BMC Neuroscience, 5(1), 4-13. https://doi.org/10.1186/1471-2202-5-4

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title = "Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors",

abstract = "Inactivating genes in vivo is an important technique for establishing their function in the adult nervous system. Unfortunately, conventional knockout mice may suffer from several limitations including embryonic or perinatal lethality and the compensatory regulation of other genes. One approach to producing conditional activation or inactivation of genes involves the use of Cre recombinase to remove loxP-flanked segments of DNA. We have studied the effects of delivering Cre to the hippocampus and neocortex of adult mice by injecting replication-deficient adeno-associated virus (AAV) and lentiviral (LV) vectors into discrete regions of the forebrain",

keywords = "Genetics",

author = "Bushra Ahmed and Sridhara Chakravarthy and Ruben Eggers and Hermens, \{Wim TJMC\} and Jing Zhang and Niclou, \{Simone P.\} and Christiaan Levelt and Fred Sablitzky and Anderson, \{Patrick N.\} and A.R. Lieberman and Joost Verhaagen",

year = "2004",

month = jan,

day = "30",

doi = "10.1186/1471-2202-5-4",

language = "English",

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pages = "4--13",

journal = "BMC Neuroscience",

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Ahmed, B, Chakravarthy, S, Eggers, R, Hermens, WTJMC, Zhang, J, Niclou, SP, Levelt, C, Sablitzky, F, Anderson, PN, Lieberman, AR & Verhaagen, J 2004, 'Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors', BMC Neuroscience, vol. 5, no. 1, pp. 4-13. https://doi.org/10.1186/1471-2202-5-4

TY - JOUR

T1 - Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors

AU - Ahmed, Bushra

AU - Chakravarthy, Sridhara

AU - Eggers, Ruben

AU - Hermens, Wim TJMC

AU - Zhang, Jing

AU - Niclou, Simone P.

AU - Levelt, Christiaan

AU - Sablitzky, Fred

AU - Anderson, Patrick N.

AU - Lieberman, A.R.

AU - Verhaagen, Joost

PY - 2004/1/30

Y1 - 2004/1/30

N2 - Inactivating genes in vivo is an important technique for establishing their function in the adult nervous system. Unfortunately, conventional knockout mice may suffer from several limitations including embryonic or perinatal lethality and the compensatory regulation of other genes. One approach to producing conditional activation or inactivation of genes involves the use of Cre recombinase to remove loxP-flanked segments of DNA. We have studied the effects of delivering Cre to the hippocampus and neocortex of adult mice by injecting replication-deficient adeno-associated virus (AAV) and lentiviral (LV) vectors into discrete regions of the forebrain

AB - Inactivating genes in vivo is an important technique for establishing their function in the adult nervous system. Unfortunately, conventional knockout mice may suffer from several limitations including embryonic or perinatal lethality and the compensatory regulation of other genes. One approach to producing conditional activation or inactivation of genes involves the use of Cre recombinase to remove loxP-flanked segments of DNA. We have studied the effects of delivering Cre to the hippocampus and neocortex of adult mice by injecting replication-deficient adeno-associated virus (AAV) and lentiviral (LV) vectors into discrete regions of the forebrain

KW - Genetics

U2 - 10.1186/1471-2202-5-4

DO - 10.1186/1471-2202-5-4

M3 - Article

SN - 1471-2202

VL - 5

SP - 4

EP - 13

JO - BMC Neuroscience

JF - BMC Neuroscience

IS - 1

ER -

Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors

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