Effect of SMMC-7721-derived exosomes on hepatocytes studied by AFM

Tuoyu Ju; Fan Yang; Ying Wang; Zhengxun Song; Hongmei Xu; Yujuan Chen; Zuobin Wang

doi:10.1109/3M-NANO49087.2021.9599805

Effect of SMMC-7721-derived exosomes on hepatocytes studied by AFM

Tuoyu Ju
, Fan Yang
, Ying Wang
, Zhengxun Song
, Hongmei Xu
, Yujuan Chen
, Zuobin Wang

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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Abstract

Exosomes play an important role in the early diagnosis and development of hepatocellular carcinoma (HCC). In the past, researchers mainly studied the contents of exosomes and the biological mechanism on cells, but the changes of mechanical properties of cells caused by exosomes are not clear. In this study, the changes of mechanical properties of hepatocytes (L-02) co-cultured with exosomes (7721-exos) derived from HCC (SMMC-7721) with low metastatic ability were studied. The 7721-exos were able to promote the proliferation and rearrange the cytoskeleton of L-02 cells. The atomic force microscopy measurement results showed that the height was increased, the adhesion and the elastic modulus were reduced. The effect was concentration dependent. The changes of cell mechanical properties induced by exosomes were of great significance for further studying the mechanism of exosomes.

Original language	English
Title of host publication	2021 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale, 3M-NANO 2021 - Proceedings
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	148-151
Number of pages	4
ISBN (Electronic)	9781665448819
ISBN (Print)	9781665448826
DOIs	https://doi.org/10.1109/3M-NANO49087.2021.9599805
Publication status	Published - 17 Nov 2021
Event	2021 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO) - Xi'an, China Duration: 2 Aug 2021 → 6 Aug 2021 http://3m-nano.org/2021/main/index.asp

Publication series

Name	2021 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale, 3M-NANO 2021 - Proceedings

Conference

Conference	2021 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO)
City	Xi'an, China
Period	2/08/21 → 6/08/21
Other	2021 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO) (02/08/2021-06/08/2021, Xi'an, China)
Internet address	http://3m-nano.org/2021/main/index.asp

Keywords

atomic force microscopy
exosomes
hepatocytes
Atomic force microscopy

ASJC Scopus subject areas

Industrial and Manufacturing Engineering
Electronic, Optical and Magnetic Materials
Metals and Alloys
Polymers and Plastics
Surfaces, Coatings and Films
Control and Optimization
Instrumentation

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10.1109/3M-NANO49087.2021.9599805

16-Effect of SMMC-7721-derived Exosomes on Hepatocytes Studied by AFMAccepted author manuscript, 522 KBLicence: CC BY-NC-ND

https://ieeexplore.ieee.org/document/9599805

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Ju, T., Yang, F., Wang, Y., Song, Z., Xu, H., Chen, Y., & Wang, Z. (2021). Effect of SMMC-7721-derived exosomes on hepatocytes studied by AFM. In 2021 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale, 3M-NANO 2021 - Proceedings (pp. 148-151). (2021 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale, 3M-NANO 2021 - Proceedings). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/3M-NANO49087.2021.9599805

Ju, Tuoyu ; Yang, Fan ; Wang, Ying et al. / Effect of SMMC-7721-derived exosomes on hepatocytes studied by AFM. 2021 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale, 3M-NANO 2021 - Proceedings. Institute of Electrical and Electronics Engineers Inc., 2021. pp. 148-151 (2021 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale, 3M-NANO 2021 - Proceedings).

@inproceedings{00371ce9297743578c9f5d5fb8dc5eef,

title = "Effect of SMMC-7721-derived exosomes on hepatocytes studied by AFM",

abstract = "Exosomes play an important role in the early diagnosis and development of hepatocellular carcinoma (HCC). In the past, researchers mainly studied the contents of exosomes and the biological mechanism on cells, but the changes of mechanical properties of cells caused by exosomes are not clear. In this study, the changes of mechanical properties of hepatocytes (L-02) co-cultured with exosomes (7721-exos) derived from HCC (SMMC-7721) with low metastatic ability were studied. The 7721-exos were able to promote the proliferation and rearrange the cytoskeleton of L-02 cells. The atomic force microscopy measurement results showed that the height was increased, the adhesion and the elastic modulus were reduced. The effect was concentration dependent. The changes of cell mechanical properties induced by exosomes were of great significance for further studying the mechanism of exosomes.",

keywords = "atomic force microscopy, exosomes, hepatocytes, Atomic force microscopy",

author = "Tuoyu Ju and Fan Yang and Ying Wang and Zhengxun Song and Hongmei Xu and Yujuan Chen and Zuobin Wang",

note = "Publisher Copyright: {\textcopyright} 2021 IEEE.; 2021 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO) ; Conference date: 02-08-2021 Through 06-08-2021",

year = "2021",

month = nov,

day = "17",

doi = "10.1109/3M-NANO49087.2021.9599805",

language = "English",

isbn = "9781665448826",

series = "2021 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale, 3M-NANO 2021 - Proceedings",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

pages = "148--151",

booktitle = "2021 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale, 3M-NANO 2021 - Proceedings",

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}

Ju, T, Yang, F, Wang, Y, Song, Z, Xu, H, Chen, Y & Wang, Z 2021, Effect of SMMC-7721-derived exosomes on hepatocytes studied by AFM. in 2021 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale, 3M-NANO 2021 - Proceedings. 2021 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale, 3M-NANO 2021 - Proceedings, Institute of Electrical and Electronics Engineers Inc., pp. 148-151, 2021 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO), Xi'an, China, 2/08/21. https://doi.org/10.1109/3M-NANO49087.2021.9599805

Effect of SMMC-7721-derived exosomes on hepatocytes studied by AFM. / Ju, Tuoyu; Yang, Fan; Wang, Ying et al.
2021 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale, 3M-NANO 2021 - Proceedings. Institute of Electrical and Electronics Engineers Inc., 2021. p. 148-151 (2021 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale, 3M-NANO 2021 - Proceedings).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Effect of SMMC-7721-derived exosomes on hepatocytes studied by AFM

AU - Ju, Tuoyu

AU - Yang, Fan

AU - Wang, Ying

AU - Song, Zhengxun

AU - Xu, Hongmei

AU - Chen, Yujuan

AU - Wang, Zuobin

PY - 2021/11/17

Y1 - 2021/11/17

N2 - Exosomes play an important role in the early diagnosis and development of hepatocellular carcinoma (HCC). In the past, researchers mainly studied the contents of exosomes and the biological mechanism on cells, but the changes of mechanical properties of cells caused by exosomes are not clear. In this study, the changes of mechanical properties of hepatocytes (L-02) co-cultured with exosomes (7721-exos) derived from HCC (SMMC-7721) with low metastatic ability were studied. The 7721-exos were able to promote the proliferation and rearrange the cytoskeleton of L-02 cells. The atomic force microscopy measurement results showed that the height was increased, the adhesion and the elastic modulus were reduced. The effect was concentration dependent. The changes of cell mechanical properties induced by exosomes were of great significance for further studying the mechanism of exosomes.

AB - Exosomes play an important role in the early diagnosis and development of hepatocellular carcinoma (HCC). In the past, researchers mainly studied the contents of exosomes and the biological mechanism on cells, but the changes of mechanical properties of cells caused by exosomes are not clear. In this study, the changes of mechanical properties of hepatocytes (L-02) co-cultured with exosomes (7721-exos) derived from HCC (SMMC-7721) with low metastatic ability were studied. The 7721-exos were able to promote the proliferation and rearrange the cytoskeleton of L-02 cells. The atomic force microscopy measurement results showed that the height was increased, the adhesion and the elastic modulus were reduced. The effect was concentration dependent. The changes of cell mechanical properties induced by exosomes were of great significance for further studying the mechanism of exosomes.

KW - atomic force microscopy

KW - exosomes

KW - hepatocytes

KW - Atomic force microscopy

UR - https://www.scopus.com/pages/publications/85123290119

U2 - 10.1109/3M-NANO49087.2021.9599805

DO - 10.1109/3M-NANO49087.2021.9599805

M3 - Conference contribution

SN - 9781665448826

T3 - 2021 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale, 3M-NANO 2021 - Proceedings

SP - 148

EP - 151

BT - 2021 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale, 3M-NANO 2021 - Proceedings

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 2021 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO)

Y2 - 2 August 2021 through 6 August 2021

ER -

Ju T, Yang F, Wang Y, Song Z, Xu H, Chen Y et al. Effect of SMMC-7721-derived exosomes on hepatocytes studied by AFM. In 2021 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale, 3M-NANO 2021 - Proceedings. Institute of Electrical and Electronics Engineers Inc. 2021. p. 148-151. (2021 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale, 3M-NANO 2021 - Proceedings). doi: 10.1109/3M-NANO49087.2021.9599805

Effect of SMMC-7721-derived exosomes on hepatocytes studied by AFM

Abstract

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Keywords

ASJC Scopus subject areas

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