Determination of optimal curing conditions for imaging single lung cancer cells by atomic force acoustic microscope

Xuan Wang; Yujing Zhao; Wenxiao Zhang; Ying Wang; Liguo Tian; Xinyue Wang; Zhengxun Song; Zuobin Wang; Dayou Li

doi:10.1109/3M-NANO.2018.8552210

Determination of optimal curing conditions for imaging single lung cancer cells by atomic force acoustic microscope

Xuan Wang
, Yujing Zhao
, Wenxiao Zhang
, Ying Wang
, Liguo Tian
, Xinyue Wang
, Zhengxun Song
, Zuobin Wang
, Dayou Li

Changchun University of Science and Technology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Citation (Scopus)

Abstract

This paper presents a method for the determination of optimal curing conditions for the imaging of single lung cancer cells by atomic force acoustic microscope (AFAM). The cellular morphology, height and surface roughness of the cells treated with different concentrations of paraformaldehyde and methanol and durations were observed using an AFAM. The experimental results showed that the A549 cells solidified with 4% paraformaldehyde for the period from 30min to 60min were close to the profiles of living cells. The cells solidified with 4% paraformaldehyde for 10min were beneficial to obtain subsurface structures.

Original language	English
Title of host publication	nan
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	83-86
ISBN (Print)	9781538662144
DOIs	https://doi.org/10.1109/3M-NANO.2018.8552210
Publication status	Published - 29 Nov 2018
Event	IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO) - Hangzhou Duration: 13 Aug 2018 → 17 Aug 2018

Conference

Conference	IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO)
City	Hangzhou
Period	13/08/18 → 17/08/18
Other	IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO) (13/08/2018-17/08/2018, Hangzhou)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

A549 cell
Atomic force acoustic microscope
cell curing
cured cell imaging

Access to Document

10.1109/3M-NANO.2018.8552210

https://ieeexplore.ieee.org/document/8552210

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@inproceedings{300b63f54e5e47fc83555cd4d6809f8c,

title = "Determination of optimal curing conditions for imaging single lung cancer cells by atomic force acoustic microscope",

abstract = "This paper presents a method for the determination of optimal curing conditions for the imaging of single lung cancer cells by atomic force acoustic microscope (AFAM). The cellular morphology, height and surface roughness of the cells treated with different concentrations of paraformaldehyde and methanol and durations were observed using an AFAM. The experimental results showed that the A549 cells solidified with 4\% paraformaldehyde for the period from 30min to 60min were close to the profiles of living cells. The cells solidified with 4\% paraformaldehyde for 10min were beneficial to obtain subsurface structures.",

keywords = "A549 cell, Atomic force acoustic microscope, cell curing, cured cell imaging",

author = "Xuan Wang and Yujing Zhao and Wenxiao Zhang and Ying Wang and Liguo Tian and Xinyue Wang and Zhengxun Song and Zuobin Wang and Dayou Li",

year = "2018",

month = nov,

day = "29",

doi = "10.1109/3M-NANO.2018.8552210",

language = "English",

isbn = "9781538662144",

pages = "83--86",

booktitle = "nan",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

address = "United States",

note = "IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO) ; Conference date: 13-08-2018 Through 17-08-2018",

}

Wang, X, Zhao, Y, Zhang, W, Wang, Y, Tian, L, Wang, X, Song, Z, Wang, Z & Li, D 2018, Determination of optimal curing conditions for imaging single lung cancer cells by atomic force acoustic microscope. in nan. Institute of Electrical and Electronics Engineers Inc., pp. 83-86, IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO), Hangzhou, 13/08/18. https://doi.org/10.1109/3M-NANO.2018.8552210

TY - GEN

T1 - Determination of optimal curing conditions for imaging single lung cancer cells by atomic force acoustic microscope

AU - Wang, Xuan

AU - Zhao, Yujing

AU - Zhang, Wenxiao

AU - Wang, Ying

AU - Tian, Liguo

AU - Wang, Xinyue

AU - Song, Zhengxun

AU - Wang, Zuobin

AU - Li, Dayou

PY - 2018/11/29

Y1 - 2018/11/29

N2 - This paper presents a method for the determination of optimal curing conditions for the imaging of single lung cancer cells by atomic force acoustic microscope (AFAM). The cellular morphology, height and surface roughness of the cells treated with different concentrations of paraformaldehyde and methanol and durations were observed using an AFAM. The experimental results showed that the A549 cells solidified with 4% paraformaldehyde for the period from 30min to 60min were close to the profiles of living cells. The cells solidified with 4% paraformaldehyde for 10min were beneficial to obtain subsurface structures.

AB - This paper presents a method for the determination of optimal curing conditions for the imaging of single lung cancer cells by atomic force acoustic microscope (AFAM). The cellular morphology, height and surface roughness of the cells treated with different concentrations of paraformaldehyde and methanol and durations were observed using an AFAM. The experimental results showed that the A549 cells solidified with 4% paraformaldehyde for the period from 30min to 60min were close to the profiles of living cells. The cells solidified with 4% paraformaldehyde for 10min were beneficial to obtain subsurface structures.

KW - A549 cell

KW - Atomic force acoustic microscope

KW - cell curing

KW - cured cell imaging

U2 - 10.1109/3M-NANO.2018.8552210

DO - 10.1109/3M-NANO.2018.8552210

M3 - Conference contribution

SN - 9781538662144

SP - 83

EP - 86

BT - nan

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO)

Y2 - 13 August 2018 through 17 August 2018

ER -

Determination of optimal curing conditions for imaging single lung cancer cells by atomic force acoustic microscope

Abstract

Conference

UN SDGs

Keywords

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