Controlled manipulation of TRAIL into single human colon cancer cells using atomic force microscope

Yingmin Qu; Jinyun Liu; Guoliang Wang; Zhengxun Song; Zuobin Wang

doi:10.1109/3M-NANO.2017.8286333

Controlled manipulation of TRAIL into single human colon cancer cells using atomic force microscope

Yingmin Qu
, Jinyun Liu
, Guoliang Wang
, Zhengxun Song
, Zuobin Wang

Changchun University of Science and Technology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

In this study, an AFM tip was used to penetrate the human colon cancer cells (SW480) in the culture medium containing pEGFP-N1-TRAIL plasmids. The trail plasmids encoded with the enhanced green fluorescent protein (EGFP) were moved into the SW480 cells through membrane holes created by the AFM probe. Following the penetration, the culture medium was changed into the RPMI1640 medium supplemented with 10% of fetal bovine serum and incubated for 24h. The expression of PEGFP-N1-TRAIL in SW480 cells was then observed by inverted fluorescence microscope. The experiment results indicate that the AFM tip can be used to penetrate the membranes of targeted cells individually.

Original language	English
Title of host publication	nan
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	345-348
ISBN (Print)	9781538610817
DOIs	https://doi.org/10.1109/3M-NANO.2017.8286333
Publication status	Published - 8 Feb 2018
Event	2017 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO) - Shanghai Duration: 7 Aug 2017 → 11 Aug 2017

Conference

Conference	2017 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO)
City	Shanghai
Period	7/08/17 → 11/08/17
Other	2017 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO) (07/08/2017-11/08/2017, Shanghai)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

atomic force microscope
TRAIL
human colon cancer cells

Access to Document

10.1109/3M-NANO.2017.8286333

https://ieeexplore.ieee.org/document/8286333

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Cite this

@inproceedings{b124f305956340098f3c48cd1f6e7f24,

title = "Controlled manipulation of TRAIL into single human colon cancer cells using atomic force microscope",

abstract = "In this study, an AFM tip was used to penetrate the human colon cancer cells (SW480) in the culture medium containing pEGFP-N1-TRAIL plasmids. The trail plasmids encoded with the enhanced green fluorescent protein (EGFP) were moved into the SW480 cells through membrane holes created by the AFM probe. Following the penetration, the culture medium was changed into the RPMI1640 medium supplemented with 10\% of fetal bovine serum and incubated for 24h. The expression of PEGFP-N1-TRAIL in SW480 cells was then observed by inverted fluorescence microscope. The experiment results indicate that the AFM tip can be used to penetrate the membranes of targeted cells individually.",

keywords = "atomic force microscope, TRAIL, human colon cancer cells",

author = "Yingmin Qu and Jinyun Liu and Guoliang Wang and Zhengxun Song and Zuobin Wang",

year = "2018",

month = feb,

day = "8",

doi = "10.1109/3M-NANO.2017.8286333",

language = "English",

isbn = "9781538610817",

pages = "345--348",

booktitle = "nan",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

address = "United States",

note = "2017 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO) ; Conference date: 07-08-2017 Through 11-08-2017",

}

Qu, Y, Liu, J, Wang, G, Song, Z & Wang, Z 2018, Controlled manipulation of TRAIL into single human colon cancer cells using atomic force microscope. in nan. Institute of Electrical and Electronics Engineers Inc., pp. 345-348, 2017 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO), Shanghai, 7/08/17. https://doi.org/10.1109/3M-NANO.2017.8286333

TY - GEN

T1 - Controlled manipulation of TRAIL into single human colon cancer cells using atomic force microscope

AU - Qu, Yingmin

AU - Liu, Jinyun

AU - Wang, Guoliang

AU - Song, Zhengxun

AU - Wang, Zuobin

PY - 2018/2/8

Y1 - 2018/2/8

N2 - In this study, an AFM tip was used to penetrate the human colon cancer cells (SW480) in the culture medium containing pEGFP-N1-TRAIL plasmids. The trail plasmids encoded with the enhanced green fluorescent protein (EGFP) were moved into the SW480 cells through membrane holes created by the AFM probe. Following the penetration, the culture medium was changed into the RPMI1640 medium supplemented with 10% of fetal bovine serum and incubated for 24h. The expression of PEGFP-N1-TRAIL in SW480 cells was then observed by inverted fluorescence microscope. The experiment results indicate that the AFM tip can be used to penetrate the membranes of targeted cells individually.

AB - In this study, an AFM tip was used to penetrate the human colon cancer cells (SW480) in the culture medium containing pEGFP-N1-TRAIL plasmids. The trail plasmids encoded with the enhanced green fluorescent protein (EGFP) were moved into the SW480 cells through membrane holes created by the AFM probe. Following the penetration, the culture medium was changed into the RPMI1640 medium supplemented with 10% of fetal bovine serum and incubated for 24h. The expression of PEGFP-N1-TRAIL in SW480 cells was then observed by inverted fluorescence microscope. The experiment results indicate that the AFM tip can be used to penetrate the membranes of targeted cells individually.

KW - atomic force microscope

KW - TRAIL

KW - human colon cancer cells

U2 - 10.1109/3M-NANO.2017.8286333

DO - 10.1109/3M-NANO.2017.8286333

M3 - Conference contribution

SN - 9781538610817

SP - 345

EP - 348

BT - nan

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 2017 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO)

Y2 - 7 August 2017 through 11 August 2017

ER -

Controlled manipulation of TRAIL into single human colon cancer cells using atomic force microscope

Abstract

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UN SDGs

Keywords

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