Complete sequence of the gene encoding the bacterioferritin subunit of Mycobacterium avium subspecies silvaticum

Arthur Hosie; Neil F. Inglis; Karen Stevenson; J.Michael Sharp

doi:10.1016/0378-1119(94)90889-3

Complete sequence of the gene encoding the bacterioferritin subunit of Mycobacterium avium subspecies silvaticum

Arthur Hosie
, Neil F. Inglis
, Karen Stevenson
, J.Michael Sharp

Moredun Research Institute

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

A gene encoding the bacterioferritin subunit (Bfr) of Mycobacterium avium (Ma) subspecies silvaticum has been cloned, sequenced and expressed. The 477-bp open reading frame codes for 159 amino acids, which were shown to share up to 92% identity with the Bfr of five bacterial genera. The recombinant Bfr exhibits serological cross-reactivity with Ma paratuberculosis antigen D, a protein of approx. 20 kDa in cell lysates of Ma paratuberculosis and Ma silvaticum and a protein of 20-22 kDa in sonicates of M. leprae.

Original language	English
Pages (from-to)	205-206
Journal	Gene
Volume	150
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(94)90889-3
Publication status	Published - 1 Jan 1994

Keywords

Genetics

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http://linkinghub.elsevier.com/retrieve/pii/0378111994908893

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title = "Complete sequence of the gene encoding the bacterioferritin subunit of Mycobacterium avium subspecies silvaticum",

abstract = "A gene encoding the bacterioferritin subunit (Bfr) of Mycobacterium avium (Ma) subspecies silvaticum has been cloned, sequenced and expressed. The 477-bp open reading frame codes for 159 amino acids, which were shown to share up to 92\% identity with the Bfr of five bacterial genera. The recombinant Bfr exhibits serological cross-reactivity with Ma paratuberculosis antigen D, a protein of approx. 20 kDa in cell lysates of Ma paratuberculosis and Ma silvaticum and a protein of 20-22 kDa in sonicates of M. leprae.",

keywords = "Genetics",

author = "Arthur Hosie and Inglis, \{Neil F.\} and Karen Stevenson and J.Michael Sharp",

year = "1994",

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doi = "10.1016/0378-1119(94)90889-3",

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T1 - Complete sequence of the gene encoding the bacterioferritin subunit of Mycobacterium avium subspecies silvaticum

AU - Hosie, Arthur

AU - Inglis, Neil F.

AU - Stevenson, Karen

AU - Sharp, J.Michael

PY - 1994/1/1

Y1 - 1994/1/1

N2 - A gene encoding the bacterioferritin subunit (Bfr) of Mycobacterium avium (Ma) subspecies silvaticum has been cloned, sequenced and expressed. The 477-bp open reading frame codes for 159 amino acids, which were shown to share up to 92% identity with the Bfr of five bacterial genera. The recombinant Bfr exhibits serological cross-reactivity with Ma paratuberculosis antigen D, a protein of approx. 20 kDa in cell lysates of Ma paratuberculosis and Ma silvaticum and a protein of 20-22 kDa in sonicates of M. leprae.

AB - A gene encoding the bacterioferritin subunit (Bfr) of Mycobacterium avium (Ma) subspecies silvaticum has been cloned, sequenced and expressed. The 477-bp open reading frame codes for 159 amino acids, which were shown to share up to 92% identity with the Bfr of five bacterial genera. The recombinant Bfr exhibits serological cross-reactivity with Ma paratuberculosis antigen D, a protein of approx. 20 kDa in cell lysates of Ma paratuberculosis and Ma silvaticum and a protein of 20-22 kDa in sonicates of M. leprae.

KW - Genetics

U2 - 10.1016/0378-1119(94)90889-3

DO - 10.1016/0378-1119(94)90889-3

M3 - Article

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VL - 150

SP - 205

EP - 206

JO - Gene

JF - Gene

IS - 1

ER -

Complete sequence of the gene encoding the bacterioferritin subunit of Mycobacterium avium subspecies silvaticum

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Keywords

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