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A study on the imobilization method of E. coli for AFM detection under physiological conditions

  • Siliang Zhang
  • , Yang Wang
  • , Litong Dong
  • , Zhibo Zhang
  • , Yujuan Chen
  • , Zuobin Wang
  • , Qiuxiang Dong
  • , Yanling Tian
  • , Cuihua Hu
  • Changchun University of Science and Technology
  • Baoding Institute for Food and Drug Control
  • University of Warwick

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

The micro-nano manipulation techniques based on Atomic Force Microscopy (AFM) is an important tool for studying the morphology and mechanical properties of bacteria under physiological conditions. However, immobilizing the bacteria on the substrate is the key obstacle for AFM detection in liquid condition. In this study, the methods of embedding and adsorption were combined to improve the immobilization efficiency. Compared to E. coli on a non-structured base surface, E. coli on a structured base surface appears more stretched out and is less likely to aggregate. It grown along the structure of the substrate, achieving nanomanipulation to a certain extent. This study will provide a new immobilization method for microorganism as well as E. coli.

Original languageEnglish
Title of host publication2024 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale, 3M-NANO 2024
EditorsYing Wang, Yuxiao Cui, Jingran Zhang, Miao Yu, Hao Wu, Zuobin Wang
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages403-406
Number of pages4
ISBN (Electronic)9798350362107
DOIs
Publication statusPublished - 3 Dec 2024
Event2024 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale, 3M-NANO 2024 - Zhongshan, China
Duration: 29 Jul 20242 Aug 2024

Publication series

Name2024 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale, 3M-NANO 2024

Conference

Conference2024 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale, 3M-NANO 2024
Country/TerritoryChina
CityZhongshan
Period29/07/242/08/24

Keywords

  • AFM
  • E. coli
  • immobilization
  • sub-microstructures

ASJC Scopus subject areas

  • Industrial and Manufacturing Engineering
  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Biotechnology
  • Electrical and Electronic Engineering

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